Measuring the rate of oxygen uptake. Objectives : 1. To demonstrate the uptake of oxygen in respiration. 2. To measure the rate at which an organism respires. 3. To learn how to set up the apparatus for respirometers. Introduction : Respirometer A respirometer is a device that been used to measure the rate of respiration of a living organism. This can be measured by calculating the rate of exchange of oxygen and carbon dioxide. A simple respirometer designed to measure oxygen uptake
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make it specific for only one type of substrate. Enzymes catalyze an unfathomable number of reactions by using a combination of only six basic mechanisms: (1) acid-base catalysis; (2) covalent catalysis; (3) metal ion catalysis; (4) electrostatic catalysis; (5) proximity and orientation effects; and (6) preferential binding of the transition state complex. Independent of the mechanistic characteristics taken to generate product‚ the initial reaction rates of every enzyme can be analyzed in order
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“Countries grow at different rates because they accumulate capital at different rates.” Is this true? Explain your answer. Since the Industrial Revolution‚ economists have attempted to explain why certain countries economies grow at greater rates than others. The post-Keynesian era saw the introduction of the Harrod-Domar model of economic growth. This model explained an economy’s growth rate by observing the level of saving and productivity of capital in the economy. The neo-classical Solow-Swann
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bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme‚ a product is produced. Enzymes are specific to their substrate‚ because the shape of their active site will only fit the shape of their substrate. It is said that the substrate is complimentary to their substrate. When the substrate binds with the enzyme‚ it forms an enzyme-substrate complex. The reaction happens immediately after and the substrate is broken down in to either two or more products
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Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A spectrophotometer can be used quantitatively
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CONCENTRATION USING UV- VISIBLE SPECTROPHOTOMETER INTRODUCTION In chemistry‚ spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light‚ near-ultraviolet‚ and near-infrared‚ but does not cover time-resolved spectroscopic techniques. Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer
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Respiration Objectives 1. 2. 3. 4. 5. 6. To describe oxidation and reduction in terms of electron and H+ transfer. To distinguish anaerobic from aerobic cellular respiration in terms of ATP‚ oxygen‚ and chemiosmosis. To demonstrate that carbon dioxide is a product of cell respiration. To determine the effect of boiling on the aerobic respiration of bean seeds and explain the result in terms of enzyme activity. To measure the rate of oxygen consumption in germinating bean seeds. To determine the
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of co-factor (MgSO₄) and the nature of substrate on the rate of cellular respiration in yeast were determined using two different set-ups. In the first set-up‚ two test tubes were used where one contains 7m and the other with 7 mL 0.2M MgSO₄ and both containing 7mL 10% yeast suspension. Here‚ data shows that the H₂O mixture showed higher amount of CO₂ evolved than MgSO₄. In the second set-up‚ six Smith fermentation tubes were used each containing different 15mL solution (starch‚ lactose‚ sucrose
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oD 4.1 Respiration Why organisms undergo respiration What is respiration and why do we need it? Respiration is the process whereby energy is released from food molecules (most usually glucose). It takes place inside living cells‚ both eukaryotic and prokaryotic. The process takes place inside the cytoplasm and the mitochondria. Energy is used to synthesise adenosine triphosphate (ATP)‚ which acts as a short-term energy store in cells. All living organisms need energy to drive their
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I. Background of the Study: Official figures show that plastics make up around 25% of the garbage that metro manila churns out‚ while an independent survey conducted by Eco waste Coalition and Greenpeace on the composition of discards on manila bay in 2006 found that 51% were plastic bags‚19% sachets and junk food wrappers‚ 5% Styrofoam‚ 1% hard plastics‚10% rubber‚ 13% biodegradable wastes (Cayetano‚ 2008;www.senatorpiacayetano.com/psc/pressreleasedetails.php?recordID=175) Biodegradable plastics
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