"Measure respiration rate in yeast using different substrate and tested with spectrophotometer" Essays and Research Papers

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    fermentation (anaerobic) or respiration (aerobic). Both of these processes involve oxidation of foodstuffs‚ yet only the latter requires oxygen. Cellular respiration is a very complex process that consists of many steps that take place inside the cell‚ in an organelle called a mitochondrion. Mitochondria are responsible for converting digested nutrients into the energy-yielding molecule adenosine triphosphate (ATP) to fuel the cell’s activities. This function‚ known as aerobic respiration‚ is the reason mitochondria

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    Bio Yeast Reaction Rates

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    3A Task 1 Aim: the aim of the experiment is to find the best temperature to ferment yeast at. Hypothesis: the yeast will ferment the best at 60 degrees Celsius. Independent variable: the temperature of the water the yeast is put in to ferment. Dependent variable. The amount of air bubbles the yeast produces. Controlled variable: the amount of yeast and glucose in each syringe. Uncontrolled variables: human error in counting. Materials : Plastic soft drink bottle cut to size Marking pen

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    Testing for Cellular Respiration by Balloon Inflation Experiment Number 2 Abstract: Through a timed test it was discovered which substance A or substance B contained glucose and when combined with yeast results in respiration; it was found that substance A contained glucose and resulted in respiration when combined with yeast. At 0 minutes all four balloon’s circumference was 9 centimeters; substance B and salt remained the same circumference‚ 9 centimeters‚ for

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    EFFECT OF GLUCOSE CONCENTRATION ON ANAEROBIC RESPIRATION IN YEAST INTRODUCTION: Yeast‚ also known as a saccharomyces cerevisiae‚ is single celled eukaryotic cells that are in the kingdom fungi and are unicellular organisms which normally reproduce asexually by budding at a very high rate. Scientists quite often decide to work with yeast because of its features fast growing rate and the fact that yeast ’s DNA can be easily manipulated. Some types of yeast can be found naturally on plant or in the soil

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    of Glucose in Yeast Cells Glucose is absorbed across the cell surface membrane (plasma membrane) of most cells. A convenient way to investigate this is to use a solution of glucose and a suspension of yeast cells. The amount of glucose taken up from the glucose solution by yeast cells in a fixed length of time can be measured. At the end of the fixed length of time‚ further uptake of glucose is prevented by transferring the yeast suspension to a boiling water bath to kill the yeast cells. If the

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    AMDG THE RATE OF PHOTOSYNTHESIS The Factor of Carbon Dioxide I. Introduction In this lab‚ the rate of photosynthesis will be measured by manipulating a variable that is needed for the process of photosynthesis. Photosynthesis is the process by which plants use light energy‚ carbon dioxide‚ and water to produce oxygen and glucose. This release of products provides the necessary nutrients for all forms of life. In water‚ leaves‚ usually float in water due to the gases such as carbon dioxide and

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    Spectrophotometer

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    Introduction What is Protein? There are more than 300 amino acids in the nature. However‚ only 20 amino acids are joined by each other with peptide bonds in different sequences and numbers. These sequences are formed by helping of genetic information which is encoded in genes. So‚ each protein molecule has its own amino acid sequence. Proteins are most abundant long chain macromolecules in all cells and they serve as structure and function matter in cells of all living organisms such as hormones

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    Spectrophotometer Lab

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    enter the spectrophotometer are clean and dry before the substrate and buffer was added. In the first experiment‚ optimal temperature for enzymatic activity was tested. Five clean spectrophotometer tubes wereare necessary with the different temperatures labeled on them using a wax pencil (Blank‚ Room Temp‚ 35 degrees Celsius‚ 45 degrees Celsius‚ and 55 degrees Celsius). Then 1mL of

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    alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature and pH for the reaction of alpha-amylase and starch was predicted to be a temperature of 50°C and a pH of 5. The optimum temperature and pH for the reaction was determined by monitoring the reaction rate of alpha-amylase at different temperatures and pH’s by means of using a spectrophotometer to measure the disappearance (in absorbance) of the substrate starch. As a result‚ the

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    Spectrophotometer

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    wavelength of maximum absorption‚ Amax‚ of bromophenol blue. 2. To construct a standard concentration curve of bromophenol blue. 3. To determine the concentration of the unknown bromophenol blue solutions. 4. To determine the concentration of two different solutes‚ bromophenol blue and methyl orange in a mixture. Materials and Methods: Refer to Biological Science practical manual page 5-11. Results: Part 1: Determination of Amax of bromophenol blue The wavelength with maximum absorbance reading

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