Proving the Beer Lambert Law Abstract: For this experiment we tested the absorbency of copper sulphate in different concentrations to prove the Beer Lambert Law. Introduction: This Law: Provides an experimental link between the absorbance properties of a solution of a given compound and its concentration. This is normally expressed as . A = Absorbance measured = Absorptivity C = Molar concentration L = Length of cuvette Equipment: • Gilson pipette • Eppendorf tubes • Copper sulphate
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First‚ the work area was sanitized with ethanol and paper towels‚ and the Bunsen-burner was lighted. The inoculating loops were sterilized by setting them above the cone of the flame to make the loop red‚ then left them to cool in the test tube tack for streaking. The media included a liquid (YED media that was made by 3g yeast extract‚ 6g anhydrous dextrose‚ 6g agar‚ and 300 mL tap water). The liquid media was on a hot plate‚ then an oven mitt was used to carefully transfer onto 4 petri dishes at
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Chemistry Lab Report (Redox Reactions) Questions: • What will happen when you put zinc in copper II sulfate? • What will happen when you put copper in copper II sulfate? Predictions: • We can predict that zinc will react with the aqueous solution of copper II sulfate and we will notice a reaction. We can also predict that the copper will not react with the solution of copper II sulfate and no reaction will be noticed. Hypothesis: • It is hypothesized the zinc metal will have a precipitation reaction
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Concentration of a Solution: Beer’s Law The objective of this experiment is to determine the concentration of an unknown copper sulfate solution. You will be using the Colorimeter. In this device‚ red light from the LED light source will pass through the solution and strike a photocell. A higher concentration of the coloured solution absorbs more light (and transmits less) than a solution of lower concentration. The Colorimeter monitors the light received by the photocell as either an absorbance
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Ion Exchange Chromatography Week 10 TAG Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column‚ load your sample and collect the hydronium ion released. 1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the
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Titration Analysis of Vinegar Student: Araik Movessian Grade: 12 Course: SCH4U Teacher: Tijana Knezovic Purpose and Introduction: In this experiment the question of “what is the molar concentration of acetic acid in a sample of vinegar?” was answered via titration using a standard solution of sodium hydroxide‚ NaOH(aq). In this investigation‚ students are to act as quality control chemists. Their purpose is to test the acetic acid concentration of the vinegar to discover whether it
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|Experiment 2. The gas produced in respiration | | | |[pic] | |(a) Label two boiling tubes A and B and place a known weight or number of living organisms in A. | | |Place an equal quantity of dead or non-living material in
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Beer’s Law Lab Hanna Beeter March 15‚ 2013 Honors Chemistry Introduction: In Beer’s law lab the purpose was to find the unknown concentration. The lab included the use of a volumetric flask‚ pipette‚ and spectrophotometer. The spectrophotometer played the biggest part in finding the concentration of the unknown. A spectrophotometer is an apparatus for measuring the intensity of light in a part of the spectrum‚ and is transmitted or emitted on a particular substance. After the number retrieved
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To investigate the effect of variation in temperature on the permeability of cell membranes using fresh beetroot Aims In this investigation‚ you will subject fresh‚ washed beetroot discs to different temperatures to investigate the effect of temperature on the permeability of the plasma membrane. Overview The beetroot discs will be placed in distilled water at different temperatures. You will compare the intensity of the beetroot pigment that has leaked out from the cells subjectively and possibly
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Bleaching of crude palm oil (CPO) Bleaching of crude palm oil will be carried out using a mass ratio of 1 synthesized adsorbent to 11 CPO. The CPO in bottle is well shaken before pouring into beaker with an amount of 110 grams. Magnetic stirrer is put into the beaker contains CPO and continuously stirred until it reaches 100 °C. After that‚ 6 drops of phosphoric acid is added into CPO followed by the addition of 10 grams synthesized adsorbent. The adsorbent must be poured slowly to ensure it mixed
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