DIFFERENT TYPES OF AGAR Mac Conkey‘s Agar plate MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at
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Agar From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Not to be confused with auger or augur. For other uses‚ see Agar (disambiguation). Culinary usage Mizuyōkan - a popular Japanese red bean jelly made from agar. Scientific usage A blood agar plate used to culture bacteria and diagnose infection. Agar or agar-agar is a gelatinous substance derived by boiling[1] a polysaccharide in red algae‚ where it accumulates in the cell walls of agarophyte and serves as the primary structural
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Title: Diffusion Rate of Methylene Blue on Agar Plates at Different Temperatures Abstract: Diffusion is the movement of particles from an area of high concentration to an area of low concentration wherein substances are independent‚ rapid and in random motion which frequently collide with each other in the sides of the container. This study aimed to assess the influence of temperature on the diffusion rate of methylene blue on agar plates. To determine the relationship between the two variables
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Problem What effect does the different concentration of hydrochloric acid have to the rate of diffusion of agar that is dissolved with sodium carbonate and phenolphthalein? Introduction Diffusion is a process where the molecules intermingle as a result of their kinetic energy of random motion and is the net movement of like molecules down their concentration gradient‚ which is the energy inherent in their constant molecular motion makes them collide and careen outward from the region of higher
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Diffusion In Agar Cubes Is Bigger Better? OR Is Smaller Smarter? Adapted from Flinn Scientific Publication No. 10110 Introduction: Diffusion is one of the very important processes by which substances such as nutrients‚ water‚ oxygen‚ and cellular wasted are transported between living cells and their environment. This activity will help you explore the relationship between diffusion and cell size by experimenting with model “cells.” In this experiment‚ you will use agar cubes to which
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unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours. Upon observing the growth on this plate‚ it is fairly obvious that one of the organisms is Serratia marcescens
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Aim To measure how different concentrations of mythelin blue affects the rate of diffusion through agar jelly. Hypothesis The diffusion of mythelin blue is directly proportional to its concentration‚ hence as the concentration increases; the rate of diffusion increases too. Controlled Variables Time time was kept constant while testing the diffusion spread of mythelin blue with each concentration. Temperature the experiment was undertaken in room temperature as change in temperature can
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differed between my group’s nutrient agar and MAC agar plates. In courgette‚ there was moderate growth in nutrient agar while it was heavy in Mac agar. In beef broth‚ there was slight growth in nutrient agar while it was negative in MAC agar. In fresh basil‚ growth was heavy in nutrient agar while it was heavy in MAC agar. Where cottage cheese was the ingredient‚ both plates had heavy growth. For walnut‚ growth was slight in nutrient agar while it was negative in MAC agar. Generally‚ the slight difference
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Diffusion in agar cubes Introduction: All cells are dependent on a processes called diffusion‚ which is the movement of a substance from an area of higher concentration to an area of lower concentration. Cells need to obtain certain essential substances‚ like oxygen‚ for their survival. The rate of diffusion is said to be dependent upon the various factors: Size of molecules that have to diffuse‚ concentration gradient‚ the distance across which diffusion occurs‚ number of pores/openings‚ surface
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growth of bacteria on Agar plates Hypothesis: That the bacteria will grow in colonies throughout the agar plates except for the one with the anti-biotic loop because some will fight off the bacteria. Method: (Method taken from prac sheet) Plate 1: Use the swab to cover your entire agar plate in your bacteria. You only need one swab of bacteria but be careful to cover the entire surface of your agar in a layer of bacteria. Carefully place an Antibiotic Mastring on top of the agar (use tweezers) in the
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