cultured cells was removed from the flask. Flask was washed with the 5 ml of 1X PBS. One ml of trypsin EDTA was added in the flask‚ and meanwhile placed in the incubator for 3-5 minutes‚ to detach the cells from the walls of flask. To normalize the trypsin EDTA‚ 5 ml of fresh media was added to the flask‚ and cells were shifted to sterilize culture tubes‚ and spun for 2 minutes at 2000 rpm. The cell pellet then obtained was re suspended in 10 ml of DMEM with antibiotics‚ into single cell suspension
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Rachel Bohnenberger Professor Van Doorn ENVS 150-002 27 April 2015 GMOs Lab Report Introduction As the population of Earth increases exponentially‚ so does the demand for more food. One of the solutions to the question of how more food can be produced is Genetically Modified Organisms‚ or GMOs. There is an ongoing debate about whether or not GMOs are more beneficial or harmful to society. Some of the benefits to GMOs are: growing plants‚ such as corn‚ that are resistant to weeds and pests‚ larger
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Purpose The purpose of the “Beanium” lab was to determine the average atomic mass of three different isotopes of beanium. During the lab beans were counted to determine mass of the sum of the beans. Each type of bean were weighed separately and then averaged together to determine the average atomic mass of beanium. Materials and Hazards Analytical balance‚ coffee filter‚ pencil‚ paper‚ cup‚ American beans‚ Nigerian beans‚ Mexican beans‚ calculator‚ and paper. Raw Data Bean Total Mass w/ Cup Number
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component‚ and so on‚ until it reaches the source again. In contrast‚ a parallel circuit is a circuit in which the has more than one resistor and has multiple paths to move along. The main purpose of this lab was to prove the laws of Ohm and Kirchhoff. On another note‚ being able to take part in this lab taught my partner and I the fundamental skills of constructing series and parallel circuit and using a multimeter to calculate the current and voltage of a circuit. I believe that if we only have the
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turn off the primary production. Darkness has no effect on respiration. This is because cellular respiration is actually the reverse process of photosynthesis. Oxygen is a necessity of life requirements for basically all living organisms.* In this lab we are testing how different levels of salinity in the water indirectly affects the gross primary productivity in aquatic plants. To measure this you would use the light and dark bottle method. Only respiration (R) can occur in the bottle stored in
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Abstract This lab is performed in order to determine the total energy in a reaction between zinc and hydrochloric acid. The reaction is done twice‚ once to measure the heat of the reaction and again to determine the work done in the system. This is because Enthalpy equals heat plus work (∆H= ∆E+W). Heat and work can be broken down further into separate components so the equation used in lab is ∆H=mc∆T + PV. Many calculations are used in the lab to find out what cannot be measured directly (ex:
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Lab report NF-κB 1. Aim The aim with this lab was through using ELISA-based technique learn how IL-1 and LPS activate the NF-κB signaling pathway in insulin-producing cells and see if the activation can be prevented through inhibition of proteasomal activity. In addition the purpose of this lab was to detect whether NF-κB activation leads to induction of the iNOS enzyme or not. 2. Background NF-κB is a transcription factor that in its inactive form is bound to IκB‚ which is a cytoplasmic inhibitory
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et al Abstract: The purpose of this lab was to determine how light affected photosynthesis‚ specifically the production of O2 bubbles. It was predicted that when the light was more intense the O2 bubble production will be high. Conversely‚ when the light was less intense the O2 bubble production will be lower. Basically the plant that is closer to the light will produce more bubbles than the plant that is placed farther away from the light source. In this lab the independent variable is the light
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FACULTY OF ENGINBERING AND BUILT ENVIRONMENT BEng (Hons) Civil Engineering Structure I Deflection Contents: Introduction 3 Objectives 3 Apparatus 4 Procedure 4 Results 4 Discussion 7 Conclusion 7 References 8 Introduction: The deflections of a beam are an engineering concern as they can create an unstable structure if they are large. People don’t want to work in a building in which the floor beams deflect an excessive amount
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daphnia should be used in the experiment • All daphnia should be collected at a same place‚ in uncontaminated waters • Experiment should be carried out in a lab at room temperature Apparatus/materials: • Pipette • Petri dish • Filter paper • Silicon grease • Needle • 0.1%‚ 0.2%‚0.3%‚0.4%‚0.5% of caffeine solutions • Daphnia culture Methodology: • Select a large specimen and‚ with a pipette‚ transfer it to the centre of a small‚ dry petri dish. with filter paper remove excess water from
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