experiment shows the effects of changing the pH level has on Catalase. As predicted‚ the farther away the pH levels got from the optimum pH (7.2)‚ the lower the reaction rate. At a pH of 7.2‚ the foam of the reaction measures 6cm. At a ph of 3 it measures 2.5 cm‚ at a pH of 5 it measures 2.75 cm‚ at ph 9 it measures 2.3 and at 11cm it measures 2cm. pH measures the hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result of altering
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Testing Catalase Activity Prepared by Anel Behric For professor Goodrow General Biology Lab Mohawk Valley Community College 10/14/2014 6:00 PM Introduction: This experiment was conducted to examine the breakdown of substrate hydrogen peroxide (H2O2) by catalase‚ which is a specific enzyme that breaks down substrates of (H2O2). Molecules are in our bodies and nature. They move around constantly which causes them to be part of random collisions‚
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launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach)‚ Brassica oleracea (Broccoli)‚ Solanum tuberosum (Russet Potato)‚ Malus domestica (Apple)‚ and Allium cepa (Onion). The five different catalases from the sources will all be used
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graph shows that the highest rate of reaction is at pH 7. This therefore supports my theory that at pH 7 the rate of reaction will be highest. This relate to other resources‚ such as books and the internet‚ which show that the optimum pH value for catalase is pH7‚ therefore there is no difference between the optimum pH and the pH used in the experiment‚ hence the enzyme structure is not affected by the pH and is most active. However the graph inadequately supports the second part of the prediction
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affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than 37℃‚ then it will break down the peroxide faster because yeast
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of hydrogen peroxide with a fixed mass of catalyst. A catalyst is a substance‚ which alters the speed‚ or rate of a chemical reaction but is chemically unchanged at the end of the reaction. The two factors that we can change are the temperature and the concentration. We chose to vary the concentration of hydrogen peroxide. The catalyst to speed up the reaction without affecting the result will be manganese oxide. Prediction: I predict that the higher the concentration of hydrogen peroxide‚ the
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enzyme; this is where the magic happens. Substrate is what is being broken apart by the enzyme. In this case‚ the enzyme is catalase and the substrate is hydrogen peroxide‚ or 2H2O2. As the catalase and the substrate interact‚ the substrate is brought into the catalase and broken apart into water and oxygen. This process may be able to happen naturally‚ but the enzyme‚ catalase‚ speeds up the process.
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decreasing the energy of activation. The main function of enzyme catalase is to convert hydrogen peroxide (H2O2) in our bodies into oxygen and water. This can be visually seen when hydrogen peroxide is put on a wound and the peroxide bubbles. Enzymes can also be found in plant cells and fungi. (Huston.) In this experiment we test the many variables that can change the rate of this reaction such as temperature‚ concentration levels of enzyme catalase and pH values. We are able to track these changes using
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unknown 2 (1 point) negative 6. unknown 3 (1 point) negative 7. unknown 4 (1 point) positive 1. Report the results from the catalase test. (3 points) 1. Liver Catalase present? Yes/No (1 point) yes 1. Roast Beef Catalase present? Yes/No (1 point) yes 1. Student supplied meat (provide name of meat): Chicken (Buddig) Catalase present? Yes/No (1 point): yes
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the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar
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