that is often described as “biting” and as “hanging” in the nasal passages. Ethanol has a slightly sweeter (or more fruit-like) odor than the other alcohols. In general‚ the hydroxyl group makes the alcohol molecule polar. Those groups can form hydrogen bonds to one another and to other compounds (except in certain large molecules where the
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substrate concentration on enzyme activity * Catalase – an enzyme that catalyses the conversion of hydrogen peroxide into water and oxygen * Source of catalase is potato: amount of oxygen produced is amount of enzyme activity * 5 test tubes‚ different amounts of H2002 and distilled water‚ measure height of bubbles of oxygen * Results: an increase in substrate concentration produces an increase of the activity of the enzyme catalase Test the effect of temp on the activity of enzymes
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Biology Honors Final Exam Review Study Guide A. Intro Unit * Characteristics of life (DR.CHARGE) * DNA * Directs protein synthesis * Reproduce * Divides in mitosis (asexual reproduction) * Cells * Homeostasis * The process by which cells maintain constant internal condition (water‚ temperature) * Adaptation (Evolution) * Respond to stimulus * Growth (Development) * Energy * Heterotroph: an organism that cannot synthesize its own food
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acidification is found to cause loss of viability of probiotic bacteria. Lactobacillus delbrueckii subsp. bulgaricus produces sufficient hydrogen peroxide to display inhibition of probiotic. Accumulation of hydrogen peroxide in growth media can happen because lactobacilli do not have catalase enzyme responsible for degradation of hydrogen peroxide to water and oxygen. Hydrogen peroxide and other toxic oxygenic metabolites such as superoxide anion and hydroxyl radical can react with other components eventually
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media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with me on the journey to the unknown. As I recall from earlier that semester‚ the first test that needed to be performed was the Gram stain. The Gram stain is
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CHAPTER ONE INTRODUCTION The industrial attachment is an important part of the course structure of the university. This is the time students appreciate the theoretical work done in the lecture halls when seen practically. This attachment introduces the student to the outside world in terms of working experience and also prepares them for the future. The department of applied biology therefore introduced the industrial attachment program in its learning curriculum to give students a chance to combine
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Abstract In food industry such as the production of cheese‚ bacteria takes a significant role on the wide variety of cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After
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capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide. The unknown bacteria was unable to break down the hydrogen peroxide‚ meaning it was catalase-negative. This result from this test correlates with the S. pyogenes and is in contradiction with S. aureus‚ a strong indication that the bacteria is in fact S.
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of antibiotics on microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol salt agar‚ blood agar and MacConkey agar plates which must be used based on the components of the bacteria. The catalase test will be used to understand the difference in facultative anaerobic and groam positive from aero tolerant anaerobes.
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Cell Biology- Osmosis‚ Cell Size and Diffusion and Enzymes 1.0 INTRODUCTION Cells are the basic building blocks of all living things. They provide structure for the body‚ take in nutrients from the food‚ convert those nutrients into energy‚ and carry out specialized functions. Cells also contain the body’s genetic material and can make copies of themselves. A cell is also a metabolic compartment where many different chemical reaction occur. There are two types of cells‚ eukaryotic and prokaryotic
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