Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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GROUP No.: | DATE: 02/14/13 | | NAME: | | EXPERIMENT No.25BACILLUS: Diagnostic Tests | | ASCOLI’S THERMOPRECIPITIN TEST Purpose: used to identify anthrax bacilli in animal hides and meat. Principle: This test was designed to detect B. anthracis antigens in the tissues of animals being utilized in animal by-products and thereby to reveal when these products contained ingredients originating from animals that had died of anthrax. The thermostable antigens involved are common to other
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INTRODUCTION The ever increasing population in the world today has led to the mounting of sewage‚ refuse and industrial waste everywhere. This waste has no place where it could be discarded and now it has found its way into the water bodies polluting the water and thereby creating a threat to the marine flora and fauna. In the environment‚ organisms are usually exposed not just to a single pollutant but rather to a mixture of these chemicals. Mumbai is one of the busiest metropolitans in the country
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FHSB1214 Unit description Biology I Year and trimester of study 2015‚ Trimester 1 Title of lab report Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition. Lecturer’s name Norkhalidah Binti Jamali Investigation of the Enzymatic Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Comments are indicated by ‘>>>’ (in detail)‚ ‘???’ (unclear) or ‘xxx’ (wrong concept) FHSB1214 Biology I (201505)-Lab Report Marking Scheme (Practical
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surface‚ it induces an immune response that involves hydrogen peroxide and nitric oxide. For example‚ when it reaches the gut‚ it induces what is clinically referred to as enterocolitis‚ or infection of the digestive tract (Suzuki‚ 1994). Methicillin-resistant S. aureus (MRSA)‚ just like other S. aureus strains avoids such immune response through catalase‚ which efficiently breaks down hydrogen peroxide into water and oxygen. This activity of the catalase in the escape of the host’s immune reaction has
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
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Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
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yellow. If there is only glucose fermentation‚ the slant will have a yellow butt. If there are both glucose and lactose fermentation‚ there will be a yellow slant and butt. Kliger’s Iron Agar slants is also used to check the presence of hydrogen sulfide. If hydrogen sulfide reacts with ferrous sulfate‚ the slant will have a black butt‚ and the test result is positive. Otherwise‚ if the slant has a yellow butt‚ the test result is negative. For unknown microorganism #17‚ the slant turned a pink color
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I had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
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