OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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Introduction : Solanum nigrum Linn. commonly known as Black Nightshade is a dicot weed in the Solanaceae family. Other names of S. nigrum are European Black Nightshade or just black nightshade‚ Duscle‚ Garden Nightshade‚ Hound’s Berry‚ Petty Morel‚ Wonder Berry‚ Small-fruited black nightshade. It is a species of the Solanum genus‚ native to Eurasia and introduced in the Americas‚ Australia‚ Asia and South Africa. The plant is cultivated as a food crop‚ both for its fruit and its leaves in some
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f) Product of the reaction In my investigation‚ I will be using the enzyme catalase‚ which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide into water and oxygen. 2H2O2 + Catalase > 2H2O + O2 Catalase dramatically reduces the activation energy needed for the reaction. Without catalase the decomposition would take much longer‚ and would not be fast enough to sustain human life. Hydrogen peroxide is also a dangerous‚ very potent by-product of metabolism‚ so it is essential
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earlier the cells were cocci with long chain arrangement. After the gram stain test I carried out a catalase test. This test was carried out to investigate if my unknown was streptococci (negative) or staphylococci (positive) and or micrococci (positive). The catalase test result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide. The next step was to examine a blood agar plate to examine the colonial morphology of hemolysis
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Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
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Active transport: requires that a cell expend energy to move molecules across a membrane against the solute’s concentration gradient (the side where it is more concentrated) Feedback inhibition: Metabolic reaction is blocked by its products. A product acts as an inhibitor of one of the enzymes in the pathway to regulate metabolism. Centriole: a structure in an animal cell composed of microtubule triplets arranged in a 9+0 pattern. An animal usually has a pair of centrioles within each of its centrosomes
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be effective as a protectant against a variety of toxic chemical agents including heavy metals (Holloway and Peterson‚ 1984). The therapeutic importance of vitamin C is immense. Vitamin C stimulate the production of enzymes super oxide dismutase‚ catalase and heat shock proteins in lymphocytes to subjects exposed to physiological oxidative stress during exercise (Khassaf et.al.‚ 2003).Presently many nutraceuticals are being used in chemotherapy. Some nutraceuticals are provided with chemotherapeutic
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**Materials : 1) Blood agar plate . 2) Mannitol Salt agar (MSA) plate. 3) DNase agar plate . 4) Novobiocin disc . 5) Inoculating loop. 6) flame ( Bunsen burner) . 7) 1N hydrochloric acid (HCl) . 8) Two slides . 9) Plasma tube. 10) 3% Hydrogen Peroxide (H2O2) . 11) One unknown plate . 12) Crystal violet. 13) Gram’s iodine . 14) Safranin. 15) Alcohol . ** Methods : An unknown labeled with number 8 was given out by the lab instructor. The goal at this point was to determine unknown gram
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produced measurable acidic byproducts? Both E. coli and S. epidermidis produced measurable acidic byproduct. H.What is the cellular role of catalase? The cellular role of a catalase is to enable the breakdown of hydrogen peroxide into water and molecular oxygen. I.Which of the organisms‚ if any‚ produced catalase? S. epidermidis is the organism that produced catalase. J.Which of the organisms‚ if any‚ produced acetoin S. epidermidis is the organism that produced acetoin.
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