For many years Hydrogen peroxide was widely used for disinfecting‚ sterilization and its antiseptic properties‚ it is cheap and easily available OTC at any drug store or grocery store. “This readily available oxidant is rapidly converted to the highly reactive hydroxyl radical that damages an array of cellular components” (Atiyeh‚ Dibo‚ and Hayek‚ 2009). “The Medicines and Healthcare Products Regulatory Agency (MHRA‚ 2014). Have issued a warning regarding its use in surgery and in deep and large
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Preliminary Experiment (4% yeast concentration) Hydrogen peroxide volume – 5 cm3 Water Volume -0 cm3 Concentration Volume- 20 vols Time in Seconds Volume of O2 (cm3) Experiment 1 Experiment 2 Average 30 95 94 94.5 60 100 100 100 90 100 100 100 120 100 100 100 150 100 100 100 180 100 100 100 Modifications The results from my preliminary experiment show that 100 cm3 of oxygen has been produced in the first 30 seconds.. This reaction is far too quick and will prevent me from analysing
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INTRODUCTION Enzymes are biological catalysts that speed up chemical reactions‚ without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a ’ball’ shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme‚ a product is produced. Enzymes are specific to their
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Decomposition of Hydrogen Peroxide by Potassium Iodide Brief Description: Two solutions are mixed resulting in an eruption of foam resembling a huge stream of toothpaste. This is the classic “Elephant Toothpaste” reaction. Purpose/Goal: Procedure presents an example of a catalyzed reaction. Explanation of Experiment: The rapid evolution of oxygen gas is produced by the following reaction: 2 H2O2 (aq) = 2 H2O (l) + O2 (g) + heat The decomposition of hydrogen peroxide in the presence
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Introduction: This experiment will investigate the factors that affect the rate of reaction of the enzyme catalyse‚ an enzyme found in food such as potato and liver. Catalyse is used to remove hydrogen peroxide from cells. The enzyme speeds up the rate of decomposition of hydrogen peroxide into water and oxygen. The reaction is: Catalyse is able to speed up the process because the enzyme lowers the activation energy of the reaction. This means that the free energy required for the reaction
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Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Apparatus & Equipment: Beaker Test tubes Either: water bath (95oC) or Bunsen burner Materials: Fresh Liver Potato cubes Manganese dioxide Hydrogen peroxide** Wood splints **Caution: Hydrogen peroxide is formed continuously as a by-product of chemical reactions in living cells; it is a very toxic (poisonous) substance. [Note: using boiling tubes may provide
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CATALASE Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in reproductive reactions. Likewise‚ catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of molecules of hydrogen peroxide to water and oxygen each second. Catalase is a tetramer of four polypeptide chains‚ each over 500 amino acids long. It contains
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4.3 (b) To investigate the effect of pH on the rate of catalase activity Teacher Notes Apparatus required per class group of 24 students Chopping board 12 Knife 12 Weigh boats 12 100 ml Graduated cylinders 36 Boiling tube 36 Syringe 12 Electronic balance 3 Dropper 12 Timer 12 Labels 36 Thermometer 12 Water bath 3 Enzyme source eg. Radishes/celery Hydrogen peroxide Range of buffer solutions pH paper Washing up liquid Disposable gloves The apparatus
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Practical: The Effect of the Enzyme Catalase from Chicken Livers on Hydrogen Peroxide [pic] Strand1: SA1‚ SA2‚ SA3 Date: Group Members: Marks: /12 (Part A) + /18 (Part B) = /30 INTRODUCTION: |There are many different reactions that are happening in our bodies all the time. There are reactions that build up molecules‚ | |synthesis/anabolic reactions‚ as well as reactions that break down molecules‚ catabolic reactions. Together
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Chemical Kinetics of Hydrogen Peroxide Decomposition Purpose The purpose of this experiment was to determine the rate equation for the catalyzed decomposition of hydrogen peroxide H2O2. Procedure The procedure for this lab can be found on pages 36 - 40 in “General Chemistry CHE111L Laboratory Manual Spring 2014.” Data Tables Carbon copies of data tables and the graph have been attached to the back of this lab report. Calculations 30 mL of
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