The polymerase chain reaction (PCR) is a biochemical technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude‚ generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis‚[1][2] PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications.[3][4] These include DNA cloning for sequencing‚ DNA-based phylogeny‚ or
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Your town | * Can you describe your town or village to me? * Tell me something about your hometown. * Where are you from? * Where is your hometown? * Where do you come from? * What is the name of the street you live on? * What kind of street do you live on? * What do you like about your town? * I live in Lahore. It is my favourite place. It is the capital of the Pakistani province of Punjab and the second largest city in the country. A 2010 government estimate now puts the population
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Pretty Woman > > Directed by Garry Marshall‚ Pretty Woman is a romantic comedy and a modernized Cinderella. The story involves the evolution of the relationship between the two protagonists‚ Vivian (Julia Roberts) and Edward Lewis (Richard Gere). In the film how a business arrangement between a business magnate and a prostitute quickly becomes a genuine loving relationship. In addition to their complex business relationship‚ Edward’s lawyer Phil (Jason Alexander) is one of many obstacles to the
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How are you supposed to trust a stranger that you have met online? Most people don’t think about the negative things that can happen when meeting a stranger until something goes wrong. Meeting a stranger for the first time can be very dangerous because it can lead to injury‚ death‚ or even getting robbed. When I was a freshman in high school‚ I had a passion for collecting basketball cards and selling them. I began buying packs of cards that had a random selection of basketball players. I would
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feminism in Pretty Woman. The quintessential romance Pretty Woman (1990) staring Julia Roberts and Richard Gere is essentially a modern day fairy tale where the underprivileged young woman meets her handsome prince and lives happily ever after. The film resonates the same narrative formula as classic fable Cinderella‚ in that the film’s protagonist Vivian (Roberts) plays a prostitute who is bought for a week by wealthy businessman Edward (Gere) for everything but the standard reason‚ sex. Pretty Woman
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Henry David Thoreau’s Where I lived‚ and What I Lived For I found Henry David Thoreau?s ?Where I Lived‚ and What I Lived For? made a very convincing argument. He has many examples to support his beliefs. Thoreau stresses the importance and value of living the simplest life nature affords‚ which I believe is as important now as it was in his day. ?Where I Lived‚ and What I Lived For? Opens with Thoreau describing how he came to live in a small‚ dilapidated cabin near Walden Pond. He speaks
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Pretty in Pink Summary Pretty in Pink is essentially a love story about a girl from the wrong side of the tracks‚ the rich high school hunk who has his eye on her‚ and the peer pressure that threatens their budding romance. Molly Ringwald plays the character of Andie Walsh‚ an unpopular poor girl living in the shabbier side of town. Andrew McCarthy portrays the role of Blane McDonnagh‚ a wealthy heartthrob who asks her out to the prom. As their romance evolves‚ both characters struggle with
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How to Talk to Anyone: 92 Little Tricks for Big Success in Relationships by Leil Lowndes‚ McGraw Hill‚ 2003 This book seemed like it could be very educational in the world of public speaking. The book is written by Leil Lowndes who I have seen appear on TV more than once conducting interviews. All of her written work has to do with connecting with people so I knew that by reading this book‚ it would be good for a report like this‚ and for my own personal skills. When I opened this book and saw
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though willing to move forward‚ still ponders about the past and thinks about the limited time that life has left. Mezzo Cammin is a look into a life that has not been fully lived and has reached a point to where time is valuable‚ almost as much as obtaining a success or self righteousness of some kind. The author uses literary devices that captivate the reader and help explain the point by expressing his feelings and thoughts through words‚ using a rhyming scheme easily‚ and effortlessly using symbols
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from the fruit he had collected‚ it was in reality contentment in enjoying the beauty surrounding him‚ and that is the true treasure that farmer should feel cheated of. The farm he was so eager to buy enraptured him‚ mainly because of how isolated it was and how at peace the buildings seemed to be with nature; the apple orchard welcomed the forest rabbits‚ and the vines had overtaken the fence. He wanted to come into ownership to keep the original owner from destroying the natural splendor of it
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