Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT CHEMISTRY LAB REPORT Name: Sri Danielle Class: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay Aim My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography. Hypothesis I think that there will be a
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Experiment 1: Viscosity of Liquids Victoria Kulczak Lab Partners: Laina Maines & Heidi Osterman Date of Lab: 2/21/11 Due Date: 2/28/11 Abstract: The goal of this experiment was to determine the viscosity of given liquids. Two different methods were employed‚ the first measures time of flow of several methanol-water solutions‚ from point A to point B. The second method involves dropping a foreign object‚ in this case a sphere‚ into a cylinder of glycerol and measuring the time it takes for it to
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Name Date Lab: Denisty – Solids and Liquids Part 1: Density Background Information: The density of an object is how much “stuff”‚ or molecules‚ a substance is made of. Density is how tightly packed the molecules are. To calculate the density of a substance we need to know the mass (weight) of the substance and the volume (how much space) of the substance. Mass is determined by weighing an object. Volume of regular shaped objects is calculated by measuring
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packed closely together‚ but they are able to move around. Hence a fluid does not have a rigid shape‚ but adapts to the contours of the container that holds it. Like a liquid a gas has no fixed shape‚ but it has little resistance to compression because there is enough empty space for the molecules to move closer. Whereas a liquid placed in a container will form a puddle at the bottom of the container‚ a gas will expand to fill the container. Although the three categories seem very well defined
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Lab Report Purpose The purpose of this lab is to determine the identity of an unknown liquid by measuring its density and its boiling point and try to match it with those solutions given in Table 2 of experiment 2. Procedure Part A In Part A‚ The main purpose was to find the determination of the density of the unknown (j41) and by doing that we had to determine volumes of the unknown liquid (j41) using three different volumetric devices which are graduated cylinder‚ pipette and burette
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COLUMN AND THIN LAYER CHROMATOGRAPHY Group 9 RAMOS‚ Sharina Joy; REYES Aina Marie; REYES Jallisa Maan; RUBIO‚ John Michael; SABINO Patricia Anne; SANTOS‚ Carlos Rafael ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components‚ the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment‚ the solid used by the students was
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The vapor pressure of a liquid is the equilibrium pressure of gas molecules from that liquid (i.e.‚ the results of evaporation) above the liquid itself. A glass of water placed in an open room will evaporate completely (and thus never reach equilibrium); however‚ if a cover is placed on the glass‚ the space above the liquid will eventually contain a constant amount of water vapor. How much water vapor is present depends on the temperature‚ but not on the amount of liquid that is present at equilibrium
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2:05 P.M. Purpose: The purpose of the experiment is to measure the densities of liquids. Specifically‚ the two liquids that were used and measured in the research were deionized water (H2O) and alcohol (C2H5OH). Methods: Density is an intensive quantity that cannot be visually identified. Therefore‚ the most straightforward approach to this problem was to record the measurements of mass and volume. For volume of each liquid‚ the instruments used were a 50ml burette‚ a 50ml graduated cylinder and a 10ml
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