Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
Premium Food coloring Color E number
Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
Premium Thin layer chromatography Chromatography Solvent
Gas Chromatography Analysis of Product Mixtures Gas Chromatography Guidelines‚ Handout. Introduction Gas chromatography is a technique used to analyze chemical compounds that can be vaporized and separated in a gas phase column. Once separated‚ the analyzed substance is passed through a detector and data is obtained. The samples that we are going to analyze are: the EtOAc from Simple distillation‚ the Fraction 1‚ Fraction 2‚ and Fraction 3 from the Fractional Distillation. Experimental Procedure
Premium Chromatography Gas chromatography Analytical chemistry
Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
Premium Thin layer chromatography Chromatography
Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin‚ acetaminophen‚ ibuprofen‚ mefanemic acid‚ and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment‚ thin-layer chromatography (TLC) will be used
Premium Thin layer chromatography Chromatography
Title: Liquid Chromatography Author: Gloria Contreras Lab Partner: Jose Montanez Instructor: Teresa Potter Date Work Performed: January 13‚ 2015 Date Submitted: January 20‚ 2015 Abstract: In this lab‚ liquid chromatography is used to separate the Red 40 and Blue 1 dyes inside of grape flavored Kool-Aid. It was determined that the 5% isopropanol will remove the Red 40 dye from the stationary phase. The 28% isopropanol will remove the Blue 1 dye from the stationary phase. The more
Premium Chromatography Solubility Chemical polarity
CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
Premium Photosynthesis Solvent Leaf
Component seeks to separate‚ identify‚ quantify and analyze the active component found in Biogesic®‚ which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore‚ this study applies thin layer chromatography (TLC) technique since it is the simplest‚ cheapest yet most effective approach in terms to the study’s objectives. The TLC method implies Rf (Rate of flow) values where it is given by the distance from origin to center of subtance spot over the
Premium Thin layer chromatography Chromatography Chemical polarity
Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
Premium Chemistry Gas chromatography Chromatography
Solubility as a Property of Matter A Lab of Chemicals‚ Chromatography‚ and Crime! Chemistry is a natural science that deals with the composition of matter and the changes it undergoes. At crime scenes‚ investigators often find unknown materials that need to be identified. If an unknown material is a mixture‚ an investigator may want to know one or two things about it: What are the ingredients of the mixture? Is the mixture found at the scene the same as a known mixture? A mixture is a collection
Premium Chemistry Chemical substance Solubility