Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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Date of Experiment October 7‚ 2012 Report Submitted: October 7‚ 2012 Title: Liquids and Solids Purpose: The purpose of this experiment is to determine the boiling point of liquids and the melting point of solids. Procedure: I got all of my materials together and set up. I poured rubbing alcohol in a beaker and rubber banded it with the thermometer. I logged the last bubble that came out of the capillary tubes. After that I crushed the acetamide and carefully put it in the capillary tube
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What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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9.1 Orbitals and Theories of Chemical Bonding 1. Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct? a) MO theory predicts that electrons are localized between pairs of atoms. b) In VB theory‚ bonding electrons are delocalized over the molecule. c) MO theory accurately describes bonding in O2 and NO‚ VB theory does not. d) VB theory can describe molecular bonding in excited states. e) MO theory is used to accurately predict
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thermometer and placed in a Thiele tube filled with oil. The tube was heated by a Bunsen burner at approximately 1 °C per minute. Melting point temperatures were recorded at the first indication of fluid and then when the sample had turned completely liquid. Two trials were completed on each sample ². The melting point of 10% naphthalene / 90% Biphenyl mixture was determined by electronic instrumentation. Two samples were prepared and placed in closed end capillary tubes and then inserted into
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Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and
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Ocean County College Department of Chemistry Liquids and Solids Date Submitted: June 21‚ 2015 Date Performed: June 19‚ 2015 Lab Section: Chem-181DL1 Course Instructor: Prof. Amal Bassa Purpose The purpose of this experiment was to determine the boiling point of isopropyl alcohol and determine the melting point of acetamide. Procedure To begin I tied the test tube and thermometer together and inserted an inverted closed-end capillary tube into the test tube. I then
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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