Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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Title:The Chromatography Process of Expo Markers Abstract:This experiment is to uncover whether or not the Expo Markers are homogeneous or heterogeneous mixtures. The experiment will conduct filter paper being slightly submerged in a container of water and inspected for pigmentation changes. In the experiment the pigments will rise up the filter paper once exposed to the water (solvent). After the experiment‚ it concludes that the mixture is a homogeneous mixture due to the chemical
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Separation Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method used for the
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Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted with the
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Introduction In order to understand what HPLC‚ that is‚ High Performance Chromatography‚ it is necessary to understand the meaning of the term chromatography itself. Chromatography Chromatography is defined as a procedure by which inert materials and drug principles encountered in pharmaceuticals preparations are separated by fractional extraction‚ adsorption‚ or ion exchange on a porous solid. Chromatography is particularly useful as a means of separating and purifying complex and closely
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Experiment 3 Liquid-Liquid Extraction Discussion The success of extracting each compound out of the solution can be seen in the IR graphs for each substance. For ethyl 4-aminobenzoate‚ the N-H‚ C=O and C-O are distinguishable in their proper wavenumber positions. The amino N-H stretch was found between 3224 cm-1 and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and
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High-performance liquid chromatography (formerly referred to as high-pressure liquid chromatography)‚ HPLC‚ is a chromatographic technique used to separate the components in a mixture‚ to identify each component‚ and to quantify each component. HPLC is considered an instrumental technique of analytical chemistry (as opposed to a gravimetric technique). In general‚ the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent. Each
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CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
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High Performance Liquid Chromatography but is also referred to as High Pressure Liquid Chromatography. It is used for separating mixtures either to analyse the mixture or to separate a required product from others in a reaction mixture. It can also be used to find the relative amounts of different components in a mixture. HPLC works along the same lines as paper chromatography. In paper chromatography a liquid (mobile phase) moves past a solid (stationary phase). In paper chromatography the stationary
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Bangabandhu Sheikh Mujibur Rahman Science and Technology University‚ Gopalganj. Title : Assignment on HPLC (High-performance /High-Pressure Liquid Chromatography) Introduction: HPLC is an advanced form of liquid chromatography used in separating the complex mixture of molecules encountered in chemical and biological systems‚ in order to recognize better the role of individual molecules by using significantly higher pressure (50–350 bar). Compounds are separated by injecting a sample
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