Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it
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The “Chromatography Instrumentation Market by Systems [LC (HPLC‚ UHPLC‚ FLASH)‚ GC‚ TLC‚ SFC‚ Others (Autosamplers‚ Detectors‚ Fraction collectors)]‚ By Consumables (Reverse phase columns‚ Syringe filters‚ Vials) & Industry - Global Forecast to 2018"‚analyzes and studies the major market drivers‚ restraints‚ opportunities‚ and challenges in North America‚ Europe‚ Asia‚ and Rest of the World. Browse 123 market data tables and 49 figures spread through 407 pages and in-depth TOC on Chromatography
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Extraction Important Peaks Concluded Compound C-5 #1 -CH‚ C=O cyclopentanone C-5 #2 -OH‚ -CH 1-hexanol D-4 #1‚2 C=O‚ -CH Cannot conclude Preparative Gas Chromatography was used to separate volatile compounds‚ which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components
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Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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reason for conducting the research is to know how the colors of our favorite candy-coated sugary rewards came to be and that method of knowing is what we call “Candy Crhomatography”. Chromatography by definition according to Webster’s dictionary means “ a method for separating the constituents of a solution (gas or liquid) by exploiting the different bonding properties of different molecules”. In this case‚ the molecules being broken down are the different molecules that make up a certain color. Colors
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Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter
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Part I: Density of Unknown Liquid Trial 1 Trial 2 Trial 3 Mass of Empty 10 mL graduated cylinder (grams) 25.55 25.56 25.55 Volume of liquid (milliliters) 8.8 8.65 8.5 Mass of graduated cylinder and liquid (grams) 30.65 30.62 30.565 Part II: Density of Irregular-Shaped Solid Mass of solid (grams) 39.537 38.515 40.975 Volume of water (milliliters) 50.01 49.9 52.4 Volume of water and solid (milliliters) 54.9 54 57 Part III: Density of Regular-Shaped
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this experiment was to obtain some experimental measurement of Vapor Liquid Equilibrium for the Ethanol – Water system to verify the literature data. For this the Ethanol – Water system was heated to a certain temperature which is called the equilibrium temperature at atmospheric pressure and equilibrium temperature and compositions of vapor and liquid were measured. At the equilibrium temperature the samples of both vapor and liquid were taken in two different test-tube and the refractive index of
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