LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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A buffer stock scheme is a form of government intervention designed to stabilize price. Governments apply buffer stock schemes to unstable markets‚ such as agriculture and commodities‚ where the ability and willingness of producers to produce fluctuates sharply. A buffer stock scheme stabilizes the price of a good by setting a ceiling/maximum and floor/minimum price for a good‚ e.g. rice. (Fig. 1). Price Band for Rice (Fig. 1) P S pmax
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1) Natural buffers are chemicals that the body releases into the blood stream to help maintain a healthy pH level. Carbon dioxide (CO2) acts as an acid by donating hydrogen ions when needed and forms carbonic acid when it dissolves in water. Carbonic acid bicarbonate is important for maintaining an acid base balance in the blood as it equalizes the pH (7.5) of the blood. All body fluids have buffers that defend the body against pH changes. A process that affects buffers in the blood is exercise
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Buffer Preparation (Gozani Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 7.0 2 242.2 150-155 pH 7.5 2 242.2 120-125 pH 8.0 2 242.2 80-85 Autoclavable. 2. EDTA 0.5 M (pH8.0) 0.5M‚ 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make
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Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Measurement of Capacitance using Arduino – An Interrupt-based Approach: Part I SAIKAT PATRA‚ SHIBENDU MAHATA Introduction Capacitive sensors are widely employed to measure various physical and chemical process parameters such as displacement‚ acceleration‚ thickness‚ force‚ pressure‚ stress‚ level‚ and humidity. The measured value of capacitance is then calibrated in terms of the process parameter for indication and/or control applications. This project presents an interrupt-based approach by
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This lab report will be detailing the steps taken and the results discovered when using spectrophotometry to determine the percentage of copper in a copper-clad penny and the thicknes of the copper layer on the copper-clad penny. After 1982‚ copper coating has been used in the creation of the penny because the cost of pure copper has increase to the point that the amount needed t omake a penny cost far more than the actual value of the penny. This lab allowed us to see just how much copper coating
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MEC 325/580 Lab Report CNC Machining Lab Report Date: March 18‚ 2013 Group Leader: Jian Wu Team Members: Group Number: G4 Regment No.: D Insturctor: CONTENTS 1.Objective and introduction. 2.Principles and practice. 3.Design and result. 4.Conclusion and recommendations. Appendices 1. Objective and introduction * Objectives Operate the CNC milling machine using G-codes‚ M-codes in a CNC program. * Equipment: Minitech CNC machine (Series 2) * Machine
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