Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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IN EMBALMING. A DEAD-END ROAD TO FORMALDEHYDE-FREE CHEMICALS.. [ONLINE] Available at: http://www.themodernembalmer.com/tannin.html. [Accessed 07 October 14]. 4. Science Lab. 2013. Material Safety Data Sheet Caffeine MSDS. [ONLINE] Available at: http://www.sciencelab.com/msds.php?msdsId=9927475. [Accessed 07 October 14]. 5.Science Lab. 2013. Material Safety Data Sheet Dichloromethane MSDS. [ONLINE] Available at: http://wcam.engr.wisc.edu/Public/Safety/MSDS/Dichloromethane.pdf. [Accessed 07 October 14]
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Anza Thomas 10/24/2013 CHE 261 (KC) Dr . McAtee Lab : Extraction of Caffiene Purpose: the purpose of this experiment is to extract caffeine from tea using solvent extraction techinques. Reference: Williamson‚ K.; Minard‚ R.; Masters‚ K. Macroscale and Microscale Organic Experiments‚ 2007‚ p. 137 to 171. Procedure: In a 50 mL beaker place 15 mL of water‚ 2.0059 g of sodium carbonate‚ and a wooden boiling stick. Bring the water to a boil on the ceramic heating plate. Remove the beaker
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Observing Onion Cells Science 8 Observing Onion Cells ***Do not write anywhere on this piece of paper!!! It will be used again this afternoon!!!***** Instructions. On a piece of paper‚ write the title (see above)‚ the date‚ your block‚ and your name. Then copy out the problem (see below). Problem. What structures can you observe in an onion cell when using a compound light microscope? Hypothesis. On your lab report‚ below your Problem‚ write a Hypothesis that will
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Abstract The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided‚ and then recrystallize the caffeine. The solvents used in the experiment were an aqueous sodium carbonate and dichloromethane (DCM). Anhydrous calcium chloride pellets were used to dry the solution and emulsion layer and the DCM was then decanted. After washing the anhydrous calcium chloride pellets with more DCM‚ the solvent was evaporated‚ leaving
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Organic Lab Report Extraction Separation of a Carboxylic Acid‚ a Phenol‚ and a Neutral Substance Purpose The objective of the experiment is to identify the three substances in the mixture and to determine the percent recovery of each from the mixture. The unknown will consist of either benzoic acid or 2-chlorobenzoic acid‚ 4-t-butyl phenol. Determine the weight of crystals and also the percent yield Introduction There are various techniques of extractions‚ solid/liquid
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the Extraction lab is to separate and purify benzoic acid‚ 2-naphthol‚ and naphthalene. These organic solids are purified by partitioning the solid in 2 immiscible solvents‚ diethyl ether and sodium bicarbonate or sodium hydroxide. II. Significance a. The significance of the Extraction lab is to purify Acetanilide benzoic acid‚ 2-naphthol‚ and naphthalene by determining the partition coefficient. This value is determined by dividing the solubility of the given solute in the extraction solvent
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The Onion’s mock press release about the MagnaSoles shoe inserts satirizes how products are marketed to customers. The article is riddled through with malapropisms‚ and the whole article is a parody of an advertisement. Sarcastic irony is used when statements are made in the article that are obviously meant to show that the opposite is true‚ and hyperbole‚ or exaggeration‚ is used to show how advertisers will try to convince people that their product is useful for many more issues than it is.
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