2. Material and methods 2.1 Sample collection The following 14 samples of Staphylococcus aureus were used in this study. All samples were obtained from food contact surfaces at three food premises in University of Putra Malaysia (UPM). Samples were collected from the surface of each material according to standard swabbing techniques. The collection was carried out during weekdays (15:00-17:00) after finished the preparation of foods. Sterile swabs were removed from the coded test tubes that contain
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Student ID# 000383032 MLT1 – Experiment 5; Task 6 Differential Staining Heidi Atkinson‚ MS Lab Experiment #5-Differential Staining Through the process of differential staining‚ there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan thereby creating very thick cell membranes
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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Methicillin Resistant Staphylococcus Aureus Staphylococcus aureus bacteria‚ commonly known as staph‚ are very common. It is "a spherical gram-positive parasitic bacterium of the genus Staphylococcus‚ usually occurring in grapelike clusters and causing boils‚ septicemia‚ and other infections." In fact‚ many people have some living on their skin all of the time and it can cause infection anywhere in the body. There are antibiotics that have been known to kill this bacterium. Unfortunately
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Methicillin-resistant Staphylococcus aureus September 22‚ 2013 Methicillin-resistant Staphylococcus aureus Methicillin-resistant Staphylococcus aureus also known as MRSA‚ is a bacteria that is resistant to many antibiotics. In the community‚ most MRSA infections are skin infections. In the medical facilities‚ MRSA causes life-threatening bloodstream infections‚ pneumonia and surgical site infections. (Methecillin-resistant Staphylococcus aureus (MRSA) infections‚ 2013) Individuals would
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The problems that arise regarding the antibiotic resistance of certain bacteria has continue to worry the health industries. One of these bacteria is the Staphylococcus aureus. It has been one of the major causes of hospital-acquired infections which has resulted to a lot of deaths each year. Description Staphylococcus aureus is a gram-positive bacteria which indicates that it has a cell wall structure with a thick layer of peptidoglycan. It is a facultative anaerobe which allows it to perform
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on
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Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of peptidoglycan where gram negative bacteria (which show up pink after the gram staining process) have a much thinner layer of peptidoglycan. One thing
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medicines are of herbal origin. Staphylococcus aureus has long been recognized as one of the most important bacteria that cause disease in humans. It is the leading cause of skin and soft tissue infections such as abscesses (boils)‚ furuncles‚ and cellulitis. Although most staph infections are not serious‚ Staphylococcus aureus can cause serious infections such as bloodstream infections‚ pneumonia‚ or bone and joint infections. Anyone can get a staphylococcus aureus skin infection. You are more likely
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Cellular and Molecular Techniques - Lab #1 BIO 349 Lab Report #1 Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how
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