In this lab‚ we tested the effect of flame on different chemical solutions. In order to do this‚ we used a wooden stick soaked in a metal and nonmetal solution‚ hovered it over a bunsen burner‚ and recorded the color that the flame changed to. The seven solutions we tested were lithium chloride‚ strontium chloride‚ calcium chloride‚ sodium chloride‚ barium chloride‚ copper chloride‚ and potassium chloride. However‚ we had another four additional solutions‚ which had unknown chemical composition
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Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify
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specially. This funnel is called as V-funnel. This test is a self-compactibility test to measure the capacity of concrete to pass into the spaces. This test determines the viscosity and passing capacity of SCC. Fresh SCC is filled to the funnel without any compaction or vibration and then the sliding cover under the funnel is opened and the filling time of all concrete in the funnel to the pail is recorded. Fig. 2 V-funnel test L-box test L-box test is used to assess the passing ability of SCC to
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MEMORIAL UNIVERSITY OF NEWFOUNDLAND FACULTY OF ENGINEERING AND APPLIED SCIENCE Engineering: 4312 Mechanics of Solids I Lab Test #4 – Torsion Test OBJECTIVES: To carry out a torsion test to destruction in order to determine for a 1020 carbon steel rod specimen: 1. The modulus of rigidity‚ 2. The shear stress at the limit of proportionality‚ 3. The general characteristics of the torque‚ angle of twist relationship. REFERENCES: 1. Hibbeler‚ R. C. "Mechanics of Materials"‚ Prentice-Hall
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VO2 Max Test Objective of the test: To work out how much oxygen can be utilized during an intense exercise test‚ the maximum capacity of oxygen used in the body Background of the test: The aim of this study was to create and evaluate a submaximal cycle ergometry test based on change in heart rate ( HR) between a lower standard work rate and an individually chosen higher work rate. In a mixed population ( n = 143) with regard to sex (55% women)‚ age (21-65 years)‚ and activity status (inactive to
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Zebrafish Embryotoxicity Test (ZET) The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1‚ 0.05‚ 0.025‚ 0.0125‚ and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations. The embryos will
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Description for Using Malaria Rapid Diagnostic Test (mRDTs) The procedure of the test as contained in the manufacturer’s instructions as approved by the National guidelines for diagnosis and treatment of malaria‚ (FMoH‚ 2011) will be followed. its reliability was futher proven by Olaniyan‚ et al.‚ (2013). The RDT provided for the study has LOT number and expiry date which must be recorded. Preferably RDT of known quality assurance will be used. The device usually contains a cassette‚ an applicator
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Cold pressor test is widely used as test for auto- nomic functions. It has been used as diagnostic test for diseases like hypertension and other cardiac auto- nomic disorders. During cold pressor test ‚ the hand or foot is immersed in cold water bath held at a con- stant temperature at 80 C. Cold water immersion activates afferent pain and temperature neurons‚ re- sulting in a centrally mediated stimulation of sympa- thetic efferent neurons. In healthy subjects ‚ the CPT causes increase
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the central analgesic activity of AVS022 and its components on hot-plate test Initial studies utilizing the hot-plate test
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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