Experiment 2 : Transport Across Membrane Name : Matrix No : Group : B Semester : 1 Date of Experiment : 05.09.2013 Lecturer’s Name : Miss OBJECTIVE To study the effects of hypotonic‚ hypertonic and isotonic solutions on plant and animal cells. INTRODUCTION In cellular biology the term membrane transport refers to the collection of mechanisms that regulate the passage of solutes such as ions and small molecules through biological membranes‚ which are lipid bilayers
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1 Experiment Membrane Transport Objectives ► Referring to energy‚ what two ways can substances enter a cell? What is active transport? What is passive transport? How is osmosis related to diffusion? How can we demonstrate active transport? How can we demonstrate Brownian movement? How can we demonstrate diffusion (2 ways)? How can we demonstrate osmosis (3 ways)? In terms of relationships between substances‚ how can we define “hypertonic”‚ “isotonic”‚ and “hypotonic”? What is the relationship
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3.1 BIOLOGICAL MEMBRANES 3.1.1 Properties of Cell Membranes • Separates living cell from its nonliving surroundings. • 8 nm thick. • Selectively permeable - allows some substances to cross more easily than others. 4.1.2 Fluid Mosaic Model • Singer and Nicolson (1972) - plasma membrane is a mosaic of proteins dispersed within lipid bilayer‚ with only bilayer‚ the hydrophilic regions exposed to water. Hydrophilic region of protein Phospholipid bilayer Hydrophobic region of protein
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Transport Across The Plasma Membrane Intracellular Fluid (ICF) – The two thirds of your body fluid contained inside body cells. (Intra = within). The cytosol of the cell. Extracellular Fluid (ECF) – Fluid outside the body cells. (extra = outside). Interstitial Fluid – The ECF in tiny spaces between cells (inter = between). Plasma- the ECF in blood vessels. Lymph- The ECF in lymphatic vessels. Solute – Any material dissolved in fluid. Solvent- The fluid a Solute is dissolved in. Concentration
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experiment is to determine the stress that various alcohols have on biological membranes. Membranes within cells are composed mainly of lipids and proteins and often serve to help maintain order within a cell by containing cellular materials. Different membranes have a variety of specific functions. One type of membrane-bound vacuole found in plant cells‚ the tonoplast‚ is quite large and usually contains water. In beet plants‚ this membrane-bound vacuole also contains a watersoluble red pigment‚ betacyanin
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Biology Experiment 2 : Transport Across Membrane Name : Umi Adiba Anis Binti Che Hamid Matrix Number : CPM0147_2013B Batch : Pre-Med May Intake 2013 Group : E Date : 19.06.2013 Lecturer’s Name : Miss Nurul Izziani Objectives : I. To study the effects of hypotonic‚ hypertonic‚ and isotonic solutions on plant and animal cells. Experiment 1 : Introduction : The purpose of doing this experiment is to investigate the effects of hypotonic‚ hypertonic‚ and isotonic solutions on plant
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Diffusion across Biological Membranes: A simulation Introduction Diffusion is the process by which collisions between molecules cause to spread apart. This movement is described as movement from an area of greater concentration to an area of lower concentration. Hence‚ diffusion continues until the molecules are equally distributed. This is to ensure that molecules have reached a state of equilibrium. Diffusion occurs spontaneously‚ no energy is involved. In cells‚ Diffusion occurs through
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BIOLOGY – TRANSPORT ACROSS MEMBRANE define diffusion and describe its role in nutrient uptake and gaseous exchange in plants and humans define osmosis and describe the effects of osmosis on plant and animal tissues define active transport and discuss its importance as an energy-consuming process by which substances are transported against a concentration gradient‚ as in ion uptake by root hairs and uptake of glucose by cells in the villi . A cell is surrounded by a layer of cell surface membrane
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from the solution to the onion cells which will become bigger. PART 2 Procedure: 1. The distilled water is removed using the filter paper; 2. A drop of 5% of sucrose solution is placed at a side of the cover slip and the solution across the epidermal layer is drawn by placing filter paper on the other side of the cover slip; 3. The onion cells are examined once again through microscope. The structure of the onion cells is drawn as observed under the microscope. Results &
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Biological Membranes Lipid Membranes • Receptors‚ detecting the signals from outside: Light Odorant Taste Chemicals A Hormones Neurotransmitters Drugs • Channels‚ gates and pumps • Electric/chemical potential Neurophysiology Energy • Energy transduction: Photosynthesis Oxidative phosphorylation • • • • • • Structure Function Composition Physicochemical properties Self-assembly Molecular models highly selective permeability barrier Internal membranes for organelles Bilayer Permeability
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