difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are by and
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Tetracycline Effectiveness on Gram Positive and Gram Negative Bacteria. INTRODUCTION: Bacteria are microbial organisms which are present in various environments. Many bacteria are good and help humans synthesize materials and assist in biological processes‚ such as digestion. However‚ some bacteria can cause harmful diseases. When harmful bacteria infects the body‚ people take antibiotics in order to suppress bacteria. Different antibiotics have different ways they suppress bacteria‚ and the efficacy of
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this lab we first need to know what gram-negative and gram-positive bacteria are and what it’s composed of. Gram-positive bacteria take up the class of bacteria that stain crystal violet in the method of bacterial differentiation. Where gram-negative bacteria are a class of bacteria that does not retain crystal violet. Instead they are counter-stained pink by Safranin‚ and because of this‚ identifying positive is possible. One of the many differences between gram-negative and gram-positive bacteria
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The purpose of this lab is to isolate a bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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INTRODUCTION The Gram Negative lab had two parts; the aim of the first part is to determine the concentration of gram negative bacteria in a water sample collected from a creek near Providence Road‚ Strickling. Gram negative bacteria have a cytoplasmic membrane‚ a thin peptidoglycan layer‚ and an additional outer membrane composed of phospholipids and lipopolysaccharides. Because gram negative bacteria have a relatively thin cell wall when compared to gram positive organisms‚ they are consequently
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚ because
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Sampling of Gram-Positive Bacteria and Antibiotic Resistance Resting We thought it would be interesting to find out the different bacteria that grow on things we use on a daily basis and the level of antibiotic resistance that those bacteria have. We chose to swab the toilet seat in the MATC women’s bathroom and the ATM machine in the commons area on campus. We were certain the ATM would be dirtier than the toilet seat since the bathrooms get cleaned every day. The first step we took in testing
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_____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the colonies formed: Yeasts: Moist‚ creamy bacteria-like‚ opaque‚ or pasty colonies on media. They reproduce by budding. (when they start budding‚ they cause infections) Molds (filamentous fungi): Fluffy cottony‚ woolly or powdery colonies on medium. They reproduce by sporulation. 2. Describe or
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