LABORATORY REPORT Activity: Enzyme Activity Name: Daniel Franco Instructor: Professor Jennifer Frere Date: 03.08.2015 Predictions Sucrase will have the greatest activity at pH 6 Sucrase will have the greatest activity at 60 °C (140 °F) Sucrase activity decreases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Each enzyme has it optimum temperature range at which it functions at an optimum efficiency. In this experiment‚ Rennin might have an optimum temperature of 37 degrees Celsius as it is found in the stomachs of young mammals. As the temperature is increased‚ the rate of reaction (time for milk to curdle) will also increase until the optimum temperature is reached (37 degrees). After reaching this set point‚ the activity of the enzyme will gradually begin to decrease and the rate of reaction will
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Biochemistry CP2085 Effect of Temperature‚ Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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LAB: Substrate Concentration Affecting the Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because
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Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity is between 23 to 25 ℃.
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INTRODUCTION Enzymes are a protein serving as a catalyst‚ a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals‚ like the different players on a ball team‚ they have different specific structures and jobs. As one ball player may be very tall and one short‚ the specific different shape of the active site on an enzyme is unique and prepares it
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In this lab the peroxidase enzyme is tested in a dormant avocado seed as well as an avocado seed undergoing the process of germination. A gas pressure will be used to test the seeds and see if the peroxidase enzyme is present in either of the seeds. A catalyst is very similar to track spikes. Spikes increase a runner’s speed‚ as a catalyst speeds up the chemical reaction time in a plant. Neither the catalyst nor shoes are changed in these actions. Enzymes are macromolecules that act like catalysts
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