Increased risk of diabetes – High carbohydrates in most no fat foods • Increased sugar intake (Sanders‚ 2014) Bibliography • Lyman‚ E. (2013). Model building lipids [Web] Retrieved from http://youtu.be/4km6BOaj9pg • Sanders‚ J (2014 February 3). Lipids [Webinar]. Retrieved from http://wgu.adobeconnect.com/p46oj5ulh8z/
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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Pre-lab 1: Identifying Carbohydrates Abstract The point of this lab is to determine if a substance contains carbohydrates such as a reducing sugar and/or polysaccharides. This will be done by using Benedict’s reagent and Iodine stain tests. Benedict’s reagent will react to reducing substances in the solution by oxidizing it and changing the structure of the reducing sugar to form a colored precipitate. The color of this precipitate can be used to determine the concentration of reducing sugars in
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Fats‚ waxes‚ monoglycerides‚ and triglycerides are all types of lipids. Triglycerides are a main form of energy for both plants and animals. Triglycerides are broken down into 3 fatty acid chains. Each fatty acid chain is further broken down into multiple groups of Acetyl CoA. Acetyl CoA molecules go through the citric acid cycle and the electron transport chain. 12 ATP are formed from each Acetyl CoA molecule. Carbon chains that are saturated with hydrogen atoms Chains are straight and
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Characterization of Saponifiable Lipids and Characterization & Isolation of Complex Lipids Jan Kevin Roxas‚ Nashiba Samad‚ Erickson Santos Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Lipids building blocks of different organic material that are water insoluble. This can be divided into simple and complex lipids that have several forms like waxes‚ triacylglycerols‚ phosphoacylgylcerols‚ sphingolipids‚ eicosanoids‚ isoprenoids and many other. To identify‚
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D_____ J_____ CARBOHYDRATES LAB May 22‚ 2013 Pre-lab Questions 1. The water in test tube No. 1 is a negative control. No reaction is expected to occur because water is not a carbohydrate. Reaction by a negative control is evidence of contamination. 2. The color changes expected for positive test results are Benedict’s test: orange to brick red Tollen’s test: metallic silver Starch iodine test: blue-black 3. Identify each of the samples in Part 1 as monosaccharide
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Lipids‚ which are also known as fats play a very important part in our bodies‚ it provides energy to producing hormones. As humans we wouldn’t be able to digest and absorb food without lipids. Eating more fat than we need can lead to weight gain but with proper amounts of lipids they are a healthy part of our diet. The main role of lipids in our body is to provide energy for muscles and body processes. Half of the fuel our body needs when at rest or with everyday activity comes from lipids. Lipids
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Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify
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Lab BCH 2333 Section: Lab 1 Carbohydrates: Separation Techniques Based on Molecular Size TA: Wednesday‚ January 16th‚ 2013 Team #4 By: Partner: Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric
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starch/ proteins/ reducing sugar/disaccharide out of 4 given samples A‚ B‚ C and D. Research Question: Out of the four given samples which sample consists of protein/ starch/reducing sugar/ disaccharide? What color changes will help to identify the contents of each sample when treated with iodine‚ biuret agent and benedicts solution? Theory and background information: What is a protein?1 Proteins are macromolecules‚ consisting of one or more chains of amino acid residues. Proteins perform
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