Specificity of Protein-Ligand Binding 1). One can conclude that Orange G. has the lowest affinity for the albumin. In the experiment the concentration of Orange G that binds to protein was a lot less than either Ponceau S. or Bromophenol blue. The ligand with the highest affinity to BSA was a little more difficult to decipher. The experiment shows that all of the 2 µL of both Ponceau S. and Bromophenol blue bind to the BSA. However‚ when 5 µL of the ligand is added the BSA is saturated and cannot
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Another word for "fats." Lipids can be more formally defined as substances such as a fat‚ oil or wax that dissolves in alcohol but not in water. Lipids contain carbon‚ hydrogen and oxygen but have far less oxygen proportionally than carbohydrates. They are found in places as diverse as egg yolks and the human nervous system and are important component of plant‚ animal and microbial membranes A fatty or waxy are organic compound that is readily soluble in nonpolar solvent (e.g. ether)
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Structures‚ Biosynthesis and Biofunctions of Iron-sulfer proteins Yiming Chen‚ Brown University‚ May 11th‚ 2011 I. Introduction Iron-sulfur proteins are the proteins which contain iron-sulfur clusters‚ like sulfide-linked di-‚ tri-‚ and tetrairon centers with various oxidative states 1. An excess of 120 distinct types of enzymes and proteins are known to contain Fe-S clusters2. Iron-sulfur proteins are known for the role of the oxidation-reduction reactions of mitochondrial electron transportation
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Lipids Biochemistry November 13‚ 2013 Lipids Lipids are families of biomolecules including fats and oils‚ waxes‚ steroids ‚ cholesterol and fat soluble vitamins. All lipids are naturally occurring compounds varying greatly in structure but share the common feature of being soluble in non polar solvents such as chloroform and ether but not in water. Lipids consist mainly of carbon and hydrogen atoms linked by non polar covalent
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Analysis of Lipid through Two-Dimensional Thin Layer Chromatography Myca Pua‚ Ramon Ramos‚ Amanda Redilas‚ Kimleigh Reyes‚ Nathaniel Sim and Clara Tamondong Group 9 2F Medical Technology Biochemistry Laboratory ABSTRACT Lipids constitute a group of naturally occurring molecules that include fats‚ waxes‚ sterols‚ fat-soluble vitamins such as vitamins A‚ D‚ E‚ and K‚ monoglycerides‚ diglycerides‚ triglycerides‚ phospholipids‚ and others. In this experiment lipid was extracted from the egg yolk
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Lab BCH2333 Section: 4 (Monday afternoon 1st week) Experiment 1: Carbohydrates Separation Techniques Based on Molecular Size Demonstrator: Date Preformed: January 26th‚ 2015 Date of Submission: February 2nd‚ 2015 Team #: Purpose The purpose of this experiment is essentially two-fold. Two of the four experimental procedures of this laboratory will be used to gain a conceptual and practical understanding of separation techniques used to isolate monomers from their respective polymers. Namely
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Lipids Lipids don’t dissolve in water contains mostly C‚H‚O (lower ratio of oxygen than do carbohydrates Types: Triglycerides‚ Waxes‚ Phospholipids‚ Sterols Triglycerides: contain glycerol molecule with three fatty acids attached‚ glycerol forms backbone different triglycerides are formed by various fatty acids that attach. Types of triglycerides: Saturated Fat‚ Unsaturated Fat‚ Monounsaturated Fat‚ and Polyunsaturated Fat Saturated Fat: all carbons are saturated with hydrogens there
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Mitochondria - Take in glucose via proteins that pass the plasma membrane and after it journey through the cytoskeleton to the mitochondria inside the cytoplasm. Endoplasmic Reticulum - Both ER types are covered in making essential cellular segments. For the most part in charge of the combination and handling of proteins that are either emitted from the cell or that end up stuck in the plasma film. The lipids made in the SER are joined with phosphorous to make phospholipids‚ the most inexhaustible
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