unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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AIDS (Acquired immune deficiency syndrome or acquired immunodeficiency syndrome) is a disease caused by a virus called HIV (Human Immunodeficiency Virus). The illness alters the immune system‚ making people much more vulnerable to infections and diseases. This susceptibility worsens as the disease progresses. HIV is found in the body fluids of an infected person (semen and vaginal fluids‚ blood and breast milk). The virus is passed from one person to another through blood-to-blood and sexual contact
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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What is AIDS? Human immunodeficiency virus (HIV) is a lentivirus (slowly-replicating retrovirus) that causes acquired immunodeficiency syndrome (AIDS)‚ a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive (1). A virus is a piece of genetic material‚ RNA or DNA‚ surrounded by a protein coat. To replicate‚ a virus must infect a cell and direct its cellular machinery to produce new viruses. A virus cannot
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HIV/AIDS The world is plagued by the HIV infection which almost always comes before AIDS. If you are HIV positive‚ that doesn’t mean you have AIDS. Having AIDS usually means that you are HIV positive. HIV (Human Immunodeficiency Virus) basically breaks down your immune system until it can’t function properly anymore. AIDS (Acquired Immune Deficiency Syndrome) never really kills anyone; instead it is a disease like pneumonia or something like that‚ that the body cannot fight because its immune system
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experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper‚ a tall clear glass‚ water‚ table salt‚ 1 tsp. measuring spoon‚ an 8 oz. measuring cup‚ pencil‚ ruler‚ 6 washable markers‚ and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown‚ teal‚ red‚ orange‚ black‚ and green. The salt solution served as the mobile phase while the chromatography paper served
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Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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