BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Introduction The purpose of this lab is to determine: the coefficient of friction between a rubber stopper and a wooden ramp the coefficient of static friction between a wooden block and a wooden ramp the coefficient of kinetic friction between a wooden block and a wooden ramp The design of this experiment was done by placing a rubber stopper / wooden block onto a wooden ramp and tilting the ramp at a certain degree to cause the stopper / block to move. The measurements of the vertical distance
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Enzymes are naturally occurring biological catalysts that are extremely efficient and specific. Enzymes accelerate the rate of a reaction by factors of at least a million as compared to the same reaction without the enzyme. Most biological reaction rates are not perceivable in the absence of the enzyme. The term enzyme was first used by a German pshysiologist Wilhelm Kühne in 1897. There are over 700 different kinds of enzymes that have been identified. Enzymes can be classified into several categories
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Enzyme Kinetics Marcos‚ Nelissa S. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ABSTRACT The rationale of the experiment is basically founded in the concept of reaction rates as affected by enzyme‚ and how the enzyme works is competed by a competitive inhibitor‚ thereby impeding the forward reaction. In this experiment‚ o-diphenol oxidase‚ an enzyme that causes the browning in fruits‚ was extracted from banana and reaction rate of this was established
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Kinetics of the Decomposition of Hydrogen Peroxide Lab Introduction: In this week’s lab experiment‚ the rate of decomposition of hydrogen peroxide forming oxygen gas will be observed and studied. Since the rate of a chemical reaction is dependent on two things; the concentrations of the reactants and the temperature at which the process is performed‚ the rate can be measured at which a reactant disappears or at which a product appears. When measuring the rate‚ the rate law will be applied. The
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Title Experiment 17 Reaction Kinetics- Determination of the Activation Energy of the Reaction Between Oxalic Acid and Potassium Permanganate. Objective To determine the activation energy of the reaction between oxalic acid and potassium permanganate. Theory and Background Activation energy is the minimum amount of energy that is required to activate atoms or molecules to a condition in which they can undergo chemical transformation or physical transport. In terms of the transition-state
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Use the knowledge of kinetics to explain each of the following statements. a. An increase in temperature at which a reaction takes place causes an increase in reaction rate . An increase in temperature means increasing the energy of the molecules present. If the molecules has more energy ‚ then more of the them will collide often with enough energy ‚ to overcome the activation energy barrier. causing the reaction to proceed more quickly. b.An addition of the catalyst increases the rate at which
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EXPERIMENT 3-CHEMICAL KINETICS: THE IODINE-CLOCK REACTION J.CHAN1 and C.CABANLIG2 1NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE 2NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE UNIVERSITY OF THE PHILIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: JANUARY 8‚ 2013 DATE PERFORMED: DECEMBER 5‚ 2012 ------------------------------------------------- ABSTRACT The kinetics of the reaction between persulfate (S2O82-)
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Lecture No. 1 Chemical Kinetics 1.1 The Rate of a Reaction Chemical Kinetics is the area of Chemistry that is concerned with the speed‚ rate or mechanism at which a chemical reaction occurs. Reaction Rate is the change in the concentration of a reactant or product with time (i.e. M/s). It measures how fast a reactant is consumed and how fast a product is formed. 1.2 WRITING RATE EXPRESSIONS Consider the following hypothetical reaction. A + 2B ( 3C + D Rate = - rate
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CHEMICAL KINETICS RESULTS AND DISCUSSION The balanced equation of the reaction between sodium thiosulfate and Hydrochloric acid is: S2O32- (aq) + 2 H+ (aq) SO2 (g) + S (s) +H2O (l) Using beakers with the same diameter was very important in conducting the experiment. If different sizes were used‚ the visibility of the “x” on the paper beneath the beaker could disappear from view too early or too late than the hypothetical time depending on the depth of the solution. It was also significant
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