efficiency. The two variables that we tested were: molar ratio of insert (RFP): vector (P1 plasmid)‚ as well as the effect of removing the stuffer from the digested plasmid. We predicted that the cells transformed without stuffer would have higher ligation efficiency than the cells transformed with stuffer. The reason for this is because the stuffer (if not removed) would cause the plasmid to ligate back into itself
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cocci shaped. Some sections of the smear were too concentrated‚ which was hard to identify the shape of the bacteria‚ while other sections were less concentrated and was easier to analyze the shape. The next step according to the result‚ will be a catalase test.
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parallel bearing surface. These specimens were perpendicular to the axial load during all the tests‚ as shown in Fig. (3.15) and (3.16). Fig. (3.15): Preparation of cube specimens Fig. (3.16): Preparation of cylinder specimens 3.6. Test procedure: This section highlights the procedures used for testing the FBLWC specimens EXPosed to compression
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The presence of carbohydrates in a solution can be determined by a qualitative test of Molisch test. Molisch test is a general‚ sensitive chemical test and positive for all kinds of carbohydrates which in free form or in combined form. The test is based on the dehydration of the carbohydrate by concentrated sulfuric acid to produce an aldehyde which condenses with two molecules of phenol‚ resulting in a coloured compound. Molisch reagent is a solution of α-napthol in 95% ethanol. The aldehydes produced
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Instructor: Cynthia Ker COST SAVINGS FOR HOSPITALS Overview Currently‚ most of the hospitals in the United States are facing crisis in their operations because of the high costs used in the maintenance of the hospitals. Therefore‚ it is without doubt that‚ there is need to change how the funds of the hospitals are maintained by encouraging the participation of the hospitals’ administrators as well as the employees to lead to reduced costs. The measures to reduce costs in the hospitals should be focused
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isomerisation‚example of reducing sugar islactose‚maltose‚glucose and fructose.All monosaccharides are capable of reducing other chemicals such as copper (II) sulphate to copper oxide.Beside that disaccharides such as maltose and lactose are reducing sugar‚however sucrose is non reducing sugar.Sugar exist in solution as an equliburum mixture of open chain and closed ring or in other words is cyclic structures.In the open chain form‚the carbon contains special bond called carbonyl carbon.In closed ring
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LS-22045 CREEP TEST DEMONSTRATOR EXPERIMENTS 1 LS-22045 CREEP TEST DEMONSTRATOR EXPERIMENTS F G A H B I C J D E LEGEND A - Temperature controller & display B - Timer C - Timer switch D - Main power switch E - Heater switch F - Dial gauge G - Beam H - Weight hanger I - Support screw J - Test area 2 LS-22045 CREEP TEST DEMONSTRATOR EXPERIMENTS OBJECTIVES To
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Formulation of a Sales Program Case 1.1 The Valley Winery Pat Waller‚ recently hired as sales manager of the San Francisco region’s chain division‚ was lamenting the problems he inherited. Despite favorable sales results for the San Francisco region‚ turnover was so severe Waller could not understand how sales increased during the past several years. He was surprised to learn the average sales rep had been with the San Francisco division of Valley Winery for only seven months and sales force
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differences can be evaluated from their ingredients itself. Table below shows the baker’s percentage of both cakes. The first cake is denoted as T1‚ while the second one is denoted by T2. T1’s recipes shows contained higher percentage of cocoa powder (86%) and eggs (144%). While T2’s recipes give higher concentration of liquid (240%)‚ butter (112%)‚ sugar (200%) and self rising flour. The different amount of liquid contributes to distinct attributes of final product. Regardless of the richness
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Electric Charge Lab John Doe Physics 1130 Oct 26th‚ 2010 Objective In this lab we will investigate some properties of electric charge‚ conductors‚ and insulators using everyday objects. Theory A charged object is any object that has an excess of negative or positive charge. Most objects are neutrally charged and we don’t normally experience their charge properties. Rubbing objects together and transferring some charge from one to the other is one way to create charged objects. Like charges
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