Grams staining method and reliability of this method. According to me‚ Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though‚ Gram is concerned that the staining process developed by him is imperfect because not all bacteria can be stained by it‚ but in my opinion‚ when we pour ethanol over the slide to wash away the dye then only definite bacteria can retain
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unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge knowledge and skill in performing laboratory techniques‚ ability to critically evaluate the information obtained from
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The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature‚ pH level‚ and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria
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an organism as one single system. Instead of analysing all the individual components that make up a cell‚ the cell is instead viewed as an interacting network of genes‚ proteins and biochemical reactions and these are studied as a whole. In 20th century‚ molecular biology was focused upon. A ‘reductionist’ approach was followed‚ in which the individual components‚ such as the cell nucleus or sugar metabolism‚ were studied in isolation. However‚ we have progressed to an era where systems biology plays
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Foster Professor Ray Ramirez BIOL 2420 December 5‚ 2014 Tuberculosis: Leading Disease Killer in the World Microbes orbit the world that is around us involving being in the air and on surfaces we may come in close contact with. In the medical terms‚ microbiology involves the study of microscopic organisms‚ bacteria‚ archaea‚ fungi‚ protozoa‚ algae‚ and multi cellular animal parasites (Baumann 3-5). It is important that we acquire knowledge of these subjects because there are many infectious diseases that
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bacteria but not the growth of gram-negatives. Lactose fermenters metabolize the lactose in the media and produce acid byproducts‚ causing a color change in the colony which is dark purple‚ almost black. Strong acid productions by organisms result in a metallic green sheen. Weaker fermentation of
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reddish brown‚ dry corky rot. Heavy infection can give rise to total loss of the tubers. Light infections can occur and are difficult to detect. Media and antibiotics for isolation of Phytophthora from diseased plant tissue and soil The Oomycetes are not true fungi‚ and therefore special techniques are required for their isolation. Most species of Phytophthora grow rather slowly in vitro compared with saprophytic fungi and bacteria. In addition‚ bacterial populations need to be kept low because they
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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Streak Plate Isolation for Obtaining Pure Cultures Data Interpretation 1- In order to unsure that you get a pure sample. 2-pure culture: laboratory culture containing a single species of organism. A pure culture is usually derived from a mixed culture (containing many species) by methods that separate the individual cells so that‚ when they multiply‚ each will form an individually distinct colony‚ which may then be used to establish new cultures with the assurance that only one type of organism
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CHAPTER I INTRODUCTION 1.1 Background Food is one of human sources of calorie‚ protein‚ fats‚ and nutrition. Yet‚ because of the highly nutritious content‚ food is susceptible to growth of microorganisms. By the presence of microorganisms in food‚ the food is more likely to have shorter shelf life. Thus‚ mostly it is resolved by the addition of antimicrobial substances to food‚ such as condiments and preservatives. Condiments and preservatives could inhibit the growth of microorganisms
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