Determining the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of
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A streak culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed
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peroxide and one thing of liver. The thought of what would happen when adding more liver to the mixture was correct because it was thought that the mixture would cause a smaller reaction and it did. If more hydrogen peroxide was then added to the liver from the previous steps then there would be a more gas releasing reaction than adding more liver. Test this and it was true that more hydrogen peroxide would cause a larger reaction. This proves that enzymes‚ such as chicken liver are reusable‚ while the
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Buoyancy Lab: Archimedes’ Principle TABLE OF CONTENTS CONTENTS …………….………….…………….……………………………..Page No. 1. Abstract…………………………….…………….………….…………….……………….. 3 2. Objective & Introduction ……….……………………………………….………………...4 3. Theory & Experimental Methods ……………………………….………………………...5 4. Results & Discussion …………………………………………………….………….............6 5. Conclusions..…………………………………………………….…………………………..7 6. References.…………………………………………………………………………..………8 7. Appendix ……………………………………………………….……………..………….…9
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Organic Lab Trial 1 Trial 2 Trial 3 Observations We added the 30ml of warm water with 4.7 grams of borax powder in to the cup labeled ‘Borax Solution’ and also added a few dots of green food coloring. We then stirred it until all the solutions were completely mixed. When this happened the borax and water turned into a watery green solution. then we added 15ml of glue in the cup labeled ‘Ball mixture’ and added the mixture we made
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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Experiment 42: Ferrocene Preparation Introduction: The purpose of the lab is to prepare ferrocene from ferrous chloride and cyclopentadiene. Ferrocene‚ an organometallic‚ is a combination of two cyclopentadienide ions with a ferrous cation‚ such that the six pi electrons binds every carbon equally to the metal forming a sandwich type structure. Ferrocene has the properties of both an activated benzene (undergoes electrophilic substitution reactions) and a ferrous ion (oxidation reaction). Glyme
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Leonardo lin 8 RAM Lab report Measuring of plerococcus 1. Background information pleurococcus is an extensive algae‚it depending on the kind of environment and produces the amount of pleuroccus depending on the enviroment. It allows you to use a small plots measuring the distribution and allocation of reality to identify relevant environmental factors organisms. 2. Aim / Research Question / Problem i am going to study about the how it affect the plant or tree‚ I am using the quadrat to measure the
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Biodegradation of Deltamethrin from S1 Fungal Isolate Janice Dsouza‚ Surajvanshikumar Suvarna‚ Seekha Parida‚ Anudurga Gajendiran and Jayanthi Abraham VIT UNIVERSITY VELLORE Abstract: Microbial biodegradation is a promising approach for recovery of environmental sites contaminated with pesticides and an effective way to remove toxicants. Deltamethrin is the most commonly used pyrethroid in agricultural practices commonly used in different geographical parts of the world. It is detected in many environmental
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Halobacterium Lab Investigation Mason Pirio 12/12/13 Period 5 Table Group 3 PS 2- Experiment Design PS 3- Conclusion Writing PS 13- Nitrogen Cycle Abstract- In this experiment we tested to see how well halobacterium grows in different levels of salinity. We found out that the higher salinity in the growth medium the better the halobacterium grows. Background Information- Halobacterium is a bacteria that is found in the great salt lake. Halobacterium is an extremeophile which means it thrives
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