Background on Genomic DNA Isolation and Purification Generally‚ all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA (by RNAses‚ salt or other methods). Choosing which method to use will depend on many selection factors including: DNA is isolated from proteins by several methods including digestion of proteins by the enzyme proteinase K. Proteins are removed subsequently by salting-out‚ organic extraction‚ or binding of the DNA to a solid-phase
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Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer’s day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people "eat with their eyes" as well as their palates. Saffron and other spices were often used to provide a rich yellow color
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Mar Biotechnol (2008) 10:242–249 DOI 10.1007/s10126-007-9057-6 ORIGINAL ARTICLE Oral Administration of Bacterially Expressed VP28dsRNA to Protect Penaeus monodon from White Spot Syndrome Virus M. Sarathi & Martin C. Simon & C. Venkatesan & A. S. Sahul Hameed Received: 8 August 2007 / Accepted: 27 September 2007 / Published online: 17 January 2008 # Springer Science + Business Media‚ LLC 2007 Abstract We explored the possibility of protecting Penaeus monodon against white spot syndrome
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September 30‚ 2012 Experiment 5‚ Preparation of Synthetic Banana Oil Introduction: This experiment prepares synthetic banana oil (isopentyl acetate) through the Fisher esterification method by combining isopentyl alcohol with acetic and sulfuric acid then heating the mixture under reflux for an hour. Esterification is a chemical reaction in which two reactants (an alcohol and an acid) form an ester as the reaction product. Observations and Data: When the sulfuric acid was combined
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distillation techniques: simple and fractional. Simple distillation was done to separate ethyl acetate—which has a usual boiling point of 77º—from a less volatile component‚ while fractional distillation was done to distinguish an ethyl acetate/n-butyl acetate mixture‚ which has boiling points of 77º and 125 º‚ respectively. Results and Discussion As previously stated the standard boiling point for ethyl acetate is 77º. The data obtained resulted with the experimental boiling point being approximately
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Title: Acids‚ Bases & Buffers Objectives: 1. To understand the acid-base chemistry. 2. To prepare and evaluate a buffer system 3. To measure the buffering capacity of two types of isotonic drinks. Introduction: There are acid-forming‚ basic forming and neutral food‚ however the acid or alkaline properties of a food is unable to judge by the actual acidity of the food itself. For example‚ citrus fruits such as lemon are acidic‚ but they are alkaline-forming when we consume and digest it. Therefore
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623 Abstract To examine the effectiveness of buffers by titrating two sets of five different solutions using HCl and NaOH and monitoring the pH change of the various solutions. The data collected shows that the buffer systems made with sodium acetate and acetic acid were effect when titrated with the strong acid and the strong base. Comparison of all the solutions shows that the concepts of buffers holds true for the results from the experimentation. Introduction The main objective of this
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in 0 % concentration of lead acetate and maximum amount of lead (0.0011 mg/gm) was found in plant treated in (0.6%) concentration of lead acetate. The percentage germination for control‚ 0.2‚ 0.4‚ 0.6 percent lead acetate treated seedling was 89%‚ 88%‚ 86.66% and 83% respectively. The length of shoot was found much less in plant treated in 0.6% concentration of lead acetate (10 cm)‚ as compared to the length of shoot in plant treated with 0% concentration of lead acetate (8 cm). Length of roots was
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Introduction: The purpose of this experiment is to derive Isopentyl acetate (banana oil) from the reaction of an alcohol and a carboxylic acid in a process called Fischer esterification (Fischer-Speier esterification). The name‚ banana oil‚ is due to banana odour released by formation of Isopentyl acetate. The alcohol and the carboxylic acid used in this experiment are Isopentyl alcohol and Glacial Acetic Acid. Both the overall reaction of Fischer esterification and the specific Fischer esterification
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separate vials‚ label and store. 13. Disassemble and clean the fractional distilling apparatus using hexane for the joints and acetone for the glassware. III. Observations O O O O Ethyl Acetate Butyl Acetate (boiling point ~ 78◦C) (boiling point ~ 120◦C) Initial temperature of fractional distillation apparatus:
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