In March 10‚ the first procedure that was done was gram staining. The purpose of this procedure was to determine if the unknown bacterium is gram-positive or gram-negative‚ and also to determine the cell shape. Gram-positive cells will be purple while gram-negative cells will be pink. The methods for the Gram staining method first starts with a fixed smear of the bacterium‚ covered it with crystal violet for 30 seconds. Afterwards‚ it should be gently rinsed off with distilled water and be covered
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The Urea test was used to test both Unknown Gram-positive and Unknown Gram-negative bacteria. One colony of bacteria was used from the Unknown 13A plate and Unknown 13B plate in order to inoculate the Urea Slant. After the slant was inoculated‚ the cultures were placed in an incubator at 35ºC for Gram-positive bacteria‚ and 37ºC for Gram-negative bacteria for a total of 48 hours. If the Urea test were positive‚ the color of the slant would have changed to a hot pink color‚ which indicated the bacteria’s
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ions. Materials: Safety glasses 10 test tubes Test tube rack Paper Pencil 50 mL beaker Bunsen burner Nichrome wire Barium Nitrate Copper Nitrate Strontium Nitrate Lithium Nitrate Potassium Nitrate Sodium Chloride Calcium Nitrate Unknown solutions A‚B‚ & C Hydrochloric Acid Wash bottle (with distilled water) Procedure: 1. Label the test tubes with the names of the solutions and unknowns. 2. Place about 5 mL of each solution into each test tube‚ and 10 mL of Hydrochloric Acid into
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solution of sodium carbonate into a conical flask. Add two or three drops of methyl orange indicator and titre this solution with KA1. Readings are recorded in the table. The titration is repeated three times to achieve accurate results. Results: Titration number | Rough | Accurate | | | 1 | 2 | 3 | Final reading/cm3 | | | | | Initial reading/cm3 | | | | | Volume of KA1/cm3 | | | | | Mass of beaker + sodium carbonate= _________________ Mass of empty beaker
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The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast
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lowest amount of energy with long wavelengths and a shorter frequency. Hypothesis: If you observe the characteristic emission spectra of several gases and
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monosaccharide opens to form a chain‚ the result can be divided into two which is an aldehyde or an ketone. Sugars that are aldehydes are known as aldoses. Sugars that are ketones are known as ketoses.Glucose and galactose are example of aldoses while fructose is an example of ketoses.There are few test can be done for testing these sugar to show or prove are they reducing sugar or non reducing sugar.Example of the test that can be done is Benedict test. 1 In plant‚glucose is
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Results and Conclusion of Research Process Paper HCS/465 September 23‚ 2012 Regan Parks Results and Conclusion of Research Process Paper In my week two assignment an article entitled Changes in fitness‚ body mass index and obesity in 9-10 year olds was used and this same article will be used again for this article. The topic for the week two paper was about the background and methodologies of this article. Now in the week three paper the topic will be about the results and conclusions of this
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1) The functional unit of the kidney is the a- Calyx b- Nephron c- Collecting duct d- Pyramid 2) All of the following are components of the nephron except the: a- Loop of Henle b- Renal corpuscle c- Proximal tubule d- Renal pelvis 3) Plasma proteins are not commonly found in the urine because: a- All proteins are subsequently reabsorbed b- All of the plasma proteins are too large to fit through the filtration slits c- All proteins filtered
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