cost-efficient‚ yet most under-utilized‚ staining method used to identify bacteria. It consists of a basic dye (the primary stain crystal violet)‚ a mordant (Gram ’s iodine)‚ decolorizer (acetone:alcohol) and a counter stain (safranin). A mordant is a compound which helps hold the primary stain to the bacteria. In this case‚ the iodine forms a complex with the crystal violet to "lock it into" the cell wall/membrane. The decolorizer is used to decolorize the bacteria that "do not like" the primary stain
Premium Bacteria Gram staining Staining
Chapter 8 Water/Minerals Sunday‚ March 24‚ 2013 6:05 PM Minerals- naturally occurring‚ inorganic‚ homogeneous substances; chemical elements. Major minerals- essential mineral nutrients required in the adult diet in amounts greater than 100 milligrams per day. Also called macrominerals. Trace minerals- essential mineral nutrients required in the adult diet in amounts less than 100 milligrams per day. Also called microminerals. Water-The brain is composed of approximately 80% water
Premium Water Human Oxygen
ABSTRACT Enzymes are highly specific and can distinguish isomers of the same molecule. The enzyme invertase specifically catalyzes the reaction of the conversion of sucrose to its individual carbohydrates glucose and fructose. It does not catalyse the reaction of maltose to 2 glucose or lactose to galactose. In this experiment‚ titrimetric and spectrophotometric methods were used to determine the specificity of invertase by determining the amount of glucose converted from the given disaccharides
Premium Enzyme Sucrose
change occur in the jar water? Because IKI was added to give it an amber color (gold color). It iodine solution and this caused the color change. 4. What does the color change in #3 indicate? Water and IKI was in jar and the iodine caused the color change of the water. 5. Why did a color change occur in the dialysis tubing bag? In the bag there was water‚ sugar (glucose) and starch. When IKI (iodine) comes into contact with the starch the contents in the bag turned blue. This IKI was able to
Premium Glucose Starch Diffusion
take advantage of the fact that different types of molecules have different functional groups and‚ therefor‚ different chemical properties. Thus‚ they will react to test reagents (also called indicators) differently. Materials: Biuret‚ Sudan III‚ Iodine‚ Benedict‚ water‚ hot plate‚ paper towels‚ pipette‚ ruler‚ goggles‚ and a timer. Method: Benedict’s test for reducing sugar: 1. Ruler was used to mark 1 cm and 2 cm marks on each tubes. 2. Negative control tube was filled with 1cm of water. Positive
Premium Chemistry Glucose Carbohydrate
and you add Bieuret reagent into each‚ then the gelatin will change color. Hypothesis F1: If you add Benedict’s solution to the urine sample and boil it‚ then it will change color‚ indicating that glucose is present. Hypothesis F2: If you add iodine to the urine sample‚ then it will turn color‚ stating that there is a starch present. Hypothesis F3: If you add Biuret’s solution to the patient’s urine sample‚ then it will change color‚ meaning a protein‚ or child‚ is present. Hypothesis F4: If
Premium Chemistry Experiment Glucose
the salt. And after the filtration the sand was left. Separation of solute between immiscible solvents * IODINE CRYSTALS + WATER + N-HEXANE the N-hexane turned to color violet. The method used to separate that mixture is Extraction. Extraction is the distribution of a solute in two immiscible solvents. The substances that separated are H2O and Iodine crystals with N-hexane. The iodine crystals have a very low solubility in water and it dissolves quickly when it combines with N-hexane and when
Premium Solubility Liquid Evaporation
Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
Premium Chemistry Bacteria Experiment
Maryely Ramirez Professor Theresa Sterner Bio Lab 105 04 November 2013 The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions‚ such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva‚ and pancreatic secretions of the small intestine. The function of amylase is
Premium PH Enzyme Amylase
Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined
Premium Solvent Chromatography Thin layer chromatography