Lanta‚ Chiqui Ann Llamado‚ Jeron Manaig College of Science Department of Biology University of Santo Tomas‚ Manila‚ Philippines Abstract Five oxygen-bearing organic compounds were given namely Methanol‚ 2-propanol‚ tert - butanol‚ formalin‚ and acetone. Different tests were done to each sample to differentiate their characteristics. These test were Dichromate test‚ Tollens Test‚ DNPH test‚ Iodoform Test‚ and Lucas Test. This was conducted to classify the samples from being a primary alcohol‚ a secondary
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INTRODUCTION Plant pigments consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most
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and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on differences
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reported establishing its antimicrobial activity (Ijeh and Ejike‚ 2011). Study by Kambizi and Afolayan (2001) showed that acetone and methanol extracts (root) of the plant possess antibacterial activity towards Bacillus cereus‚ B. pumilus‚ B. subtilis‚ Micrococcus kristinae‚ Staphylococcus aureus‚ Enterobacter cloacae and E. coli growth with MIC ranging from 0.5 – 5 (mg/ml) with the acetone extract showing higher activity. Chromatographic separation of the leaf ethanol extract by Erasto et al. (2006) yielded
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solvent and requires the presence of a strong nucleophile. For this experiment‚ you will use sodium iodide (NaI) in acetone. You will test nine substrates (Figure 1) under each of these two reaction conditions to see which compounds will do SN1‚ SN2‚ neither reaction or both. You will observe the formation of a precipitate upon reaction. For the SN2 reaction‚ NaI is soluble in acetone but the byproducts of reaction‚ NaCl or NaBr (depending on the leaving group of each compound) are not. For the
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ultraviolet-visible spectrum of this solution and analyze it for evidence of isomerization. Calculation List of Materials 4.0g tomato paste screw cap vial Chromatography column 25ml buret 50ml flask ring stamd and clamp 50% mixture of acetone and low boiling petroleum ether 5ml extraction solvent 20ml 10% aqueous solution and 20ml of saturated sodium chloride Procedure PEPARING CHROMATOGRAPHY COLUMN Pack a column for chromatography- ref. OP-21a clamp column to ring stand over 25ml
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ISOLATION OF TRIMYRISTIN FROM NUTMEG ABSTRACT The purpose if the experiment was to extract the Trimyristan from ground nutmeg using extraction‚ distillation and recrystallization procedures. This experiment involves extraction and separation techniques that yield a purified product. The product was extracted and a distillation process was used to remove any remaining solvent. The recrystallization of the sample in turn produced a pure sample‚ which was proven using the known melting
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Lab #3 Separating Metal Cations by Paper Chromatography Introduction/Purpose: In chemistry‚ it is important to effectively analyze the components in a given mixture. In order to do so‚ chemists will analyze a mixture either qualitatively or quantitatively using different methods and equipment. Qualitative analyses are subjective‚ and only gather “non-numerical” data such as the color‚ appearance‚ and smell of the components. This data
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the tannins from the coffee. Afterwards an extraction is used to separate the organic layer (bottom) from the aqueous layer (upper). By using a rotary evaporator‚ it evaporates the organic solvent and forms the crude caffeine. Afterwards we used acetone-petroleum ether for recrystallization and the vacuum apparatus for filtration to obtain pure caffeine. At the end the melting point of the pure caffeine was obtained. (Reference: Organic Chemistry Laboratory Manual) Experiment Scheme: We
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During the lab experiment six different test were conducted. Each test consists of chemicals to be tested using 3 to 10 drops of the additive in order to observe a change. With any experiment you compose a hypothesis; where you make an educated guess. The hypothesis will primarily be‚ which base chemical will produce the greatest change based on the additive. Experiment 6.1 (Starch) when adding the three drops of Iodine to each tube observe the color change. When performing this test the chemical
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