Lecture 10 Gene cloning BIOLOGY Campbell‚ Reece and Mitchell Chapter 20 Gene cloning technology Also known as: Genetic engineering or Genetic manipulation (GM) technology – implies precision engineering being applied to DNA molecules Recombinant DNA technology - implies that new combinations of DNA molecules can be made I.e. “recombinant” DNA molecules Overview of Genetic Engineering procedure 1. Making recombinant DNA molecules that can replicate in bacterial cells Genetic engineering
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Cloning of plasmid pUC19 in E.coli bacteria Introduction One aspect of the DNA cloning experiments that is carefully considered is the selection of cloning vectors. A variety of vectors have been created‚ each being suitable for a particular use. One common vector used in laboratories is a plasmid called pUC19. It is 2686 base pairs long and possesses an origin of replication which allows the production of over 100 copies in a competent E.coli cell. It possesses a multiple cloning site (MCS)
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Gene Cloning Clone: A group of identical cells or organisms To clone a gene: 1) Insert the gene into a vector that can carry the into a host cell 2) Ensure that it will replicate there 3) Insertion is carried out by cutting the vector and the DNA to be inserted with the same restriction endonuclease to ensure that both have the same "sticky ends" Vector for cloning the bacteria come from two major types: (1) Plasmids‚ and (2) Phages Recombinant DNA Technology A) It was developed in
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Gene Cloning Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from
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The technique of gene cloning has important applications in medicine‚ biotechnology‚ agriculture and research. Discuss two applications of this technique‚ which in your opinion‚ have had significant impact on society. Gene cloning is achieved by inserting a required gene into a vector DNA. This produces Recombinant DNA and when introduced to a host cell the vector is copied. It divides to produce lots of copies of the gene of interest (Willey‚ Sherwood and Woolverton‚ 2012). There are many applications
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tools and machines to do tasks more efficiently. We use technology to control the world we live in. Since the art of making fire and creating handcrafted tools‚ our civilization has come a long way. Science and Technology are making advances at an astonishing rate. From telephones to the Internet‚ calculators to computers‚ cars to rockets and satellites‚ we are part of a new world of discoveries and inventions made possible by Science. Fields like Medicine and communications have changed our cultures
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What’s the Deal with Human Cloning? Can you imagine living in a world where everyone looked the same? Picture our world without the physical diversity‚ each one of us would be uniformed. Where would we find our identity and what would set us apart? The rise of human cloning research alludes to a world where scientists can create genetic copies of human beings. The discussion of human cloning is important for you to learn about because the topic directly relates to the scientific research and technology
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TERM PAPER [pic] CLONING Submitted by:- Faculty Guide :- Atul Chikara Dr. Vipin Rajawat B.tech+M.tech biotech dual degree Amity Institute of Biotechnology Roll no - 120 Acknowledgement I have beeen greatly profited from this training and generouly lavished in this course knowledgeable interaction with such great people . I express my gratitude and dedicate this page to all
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it to happen and work properly in the society. One is human cloning. But first let’s define cloning. Cloning is the artificial process of making a genetic twin of a person or an animal. In short‚ you replicate the genes of a being in order to create another being of the same kind. And you know Dolly‚ the sheep? Dolly was the first animal to be cloned. And since her birth‚ the idea of human cloning has been given emphasis. People say cloning should not be allowed because it’s unsafe‚ it’s disadvantageous
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Biology Molecular biology Cloning AOI: AtL‚ HI‚ C&S Teacher: Elvira Kukuljac Class: I2 MYP ¸ Cloning is process of creating object which has exact genetic material as another one.1 It is very interesting field of biology for scientists as well as other people‚ but we still do not know much about how humans can be cloned. Method of cloning is very simple when it comes to
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