importance of microbiology includes: used in biomedical research‚ creation of medicines‚ environmental applications and new research tools. Disease causing organisms include: protists‚ bacteria‚ viruses and other microorganisms. Bacteria are important for fixing N2 in a usable form for plants. Bacteria and some fungi are important in decomposition and recycling of raw materials. Industry applications of microbiology: waste management‚ food industry‚ mining‚ medicine‚ research and
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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Effectiveness on Gram Positive and Gram Negative Bacteria. INTRODUCTION: Bacteria are microbial organisms which are present in various environments. Many bacteria are good and help humans synthesize materials and assist in biological processes‚ such as digestion. However‚ some bacteria can cause harmful diseases. When harmful bacteria infects the body‚ people take antibiotics in order to suppress bacteria. Different antibiotics have different ways they suppress bacteria‚ and the efficacy of antibiotics depends
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials
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Diffusion in agar cubes Introduction: All cells are dependent on a processes called diffusion‚ which is the movement of a substance from an area of higher concentration to an area of lower concentration. Cells need to obtain certain essential substances‚ like oxygen‚ for their survival. The rate of diffusion is said to be dependent upon the various factors: Size of molecules that have to diffuse‚ concentration gradient‚ the distance across which diffusion occurs‚ number of pores/openings‚ surface
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1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a sample of different
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2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test‚ an oxidase test‚ a gram stain‚ a hanging drop slide‚ and morphology identification. The unknown bacterium‚ which was contaminated with Serratia marcescens‚ was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria‚ had the
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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