The purpose of this experiment was to identify one ketone with Thin Layer Chromatography and one using NMR spectrometry. We will do this by making 2‚ 4 a DNPH derivative and checking the melting points. Theory: By Using specific methods of compounds detection‚ we can match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment‚ identifications of the unknown ketone was accomplished using thin layer chromatography‚ melting
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conducted to determine the identity of an unknown aldehyde or ketone. The tests include a Tollens test‚ a Schiff test‚ an iodoform test‚ and a derivative melting point test. The data of the first three tests was inconclusive. The final derivative melting point test was utilized to successfully determine that the unknown was the ketone‚ Propiophenone. Introduction: Qualitative analysis is a method or series of methods used to determine the identity of an unknown compound (1). It is imperative that
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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I. Title Identification of an Unknown Plasmid In this experiment‚ we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis
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EXPERIMENT 1 IDENTIFICATION OF UNKNOWN COMPOUNDS Date of experiment : 7th August 2012 Venue: ME204 INTRODUCTION Chemical reactions are regularly categorized into 3 types: oxidation-reduction (redox reaction)‚ precipitation (double displacement) and acid-base reaction (double displacement) Type 1: Oxidation-Reduction Reactions Oxidation-reduction processes include the movements of electrons form oxidants to reductants‚ which lead to increases in oxidation
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Identification of Unknown Organic Compound by Melting Point (M.P)‚ Boiling Point (B.P) and Infrared Spectroscopy Methods and Background The main objective of this lab is to identify the given unknown organic compounds with various methods. Identification of an unknown compound is important to perform through the process of melting point (M.P)‚ boiling point (B.P) and Infrared spectroscopy (I.R). Index of Hydrogen deficiency (IHD) and elemental analysis makes an experiment more efficient in
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The Identification of Two Unknown Species of Bacteria in Tube #72 Introduction: There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an
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become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect
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MIC 230 Unknown Identification Report The objective of this experiment was to identify an organism from a mixture of two unknown bacterial species. In order to accomplish this‚ I first plated my unknown mixture on Tryptic Soy Agar (TSA)‚ Columbia Naladixic Acid (CNA)‚ and MacConkey’s Agar (MAC) plates. After 48 hours of incubation‚ it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However‚ it was apparent that I had successfully
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Experiment H: Identification of an Unknown Organic Compound The objective of this lab was straightforward. We were given an unknown compound and we were to perform an IR spectroscopy and as well as NMR spectroscopy. With the IR spectroscopy‚ I was able to name the functional groups I have on my compound and further confirmed my assumptions by looking at the NMR spectroscopy after. The unknown number I was given was number 203. The molecular weight of the compound was 121. From the molecular weight
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