bactericidal effect of silver nanoparticles. Nanotechnology 16:2346–2353 Pal S‚ Tak YK‚ Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the J Nanopart Res (2008) 10:1343–1348 nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73:1712–1720 ´ˇ ´ ´ˇ ˇ ˇ ´ ´ ´ Panacek A‚ Kvıtek L‚ Prucek R‚ Kolar M‚ Vecerova R‚ Pizurova ˇ N‚ Sharma VK‚ Tat’jana N‚ Zboril Z (2006) Silver colloid nanoparticles: synthesis‚ characterization
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inside the root canal after biomechanical procedures was presented in table1. All the irrigants significantly reduced bacteria in comparison with positive control group (P< 0.01). The average reduction of bacteria in the presence of E.galbie did not have any significant difference with 2.5% NaOCl‚ CHX 2%‚ CHX 0.2% and M.communis L. (P > 0.05). The average reduction of bacteria in the presence of M.communis L. was significantly less than other groups except E.galbie and 0.2% CHX. Table1. Overall
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(TLC) and consistent with its IR & 1HNMR spectra. The synthesized analogue was screened for antibacterial activity against one gram positive & two gram negative species. The compound exhibited good antibacterial effect towards gram negative species when compared to the standard ciprofloxacin. At the same time the analogue was retaining antibacterial activity towards gram positive species when compared to standard ciprofloxacin. The molecular docking studies showed a good correlation between their
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streptomycin. Using an inoculation loop‚ E. coli was transferred from the test tube to the agar plate. This was done to each plate twice‚ creating a grid-like pattern of bacteria growth. A small paper disk soaked in either water‚ triclosan‚ or streptomycin was added to the center of the appropriate plate. To prevent the contamination of bacteria between plates and tubes‚ the inoculation loop was sterilized using an open flame. The bench all procedures were completed on was also sprayed with bleach before
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Staphylococcus aureus‚ klebsiella species‚ Escherichia coli and Salmonela paratyphi). The zone of inhibitions shown by Acetone soluble (PPAs) and acetone insoluble (PPAins) extract when compared to that of standard antibiotic (streptomycin)‚ in which gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) were found effective in ubiquitos of samples (10%PPAs and 15%PPAins)‚ however klebsiella spp. were effective on 15%PPAins only. Brine Shrimp Lethality test was performed on nauplii from which the
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1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates fermentation (test for gram -) ( glucose
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S. aureus is not diagnostic of the species. Some strains produce the hemolysin‚ while others don’t. 5. B. Alpha hemolytic and small. (There is a greenish color of the red blood cells around the small colonies) 6. B. Perform a Gram stain. 7. D. Gram-positive cocci in chains. 8. C. Catalase test. 9. B. Streptococcus 10. B. Optochin (P disk) 11. this test is used to differentiate different types of alpha-hemolytic streptococci. 12. A. It is viridans streptococci
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"NATURAL PRESERVATIVES" Anthony C. Dweck Research Director‚ Peter Black Medicare Ltd.‚ White Horse Business Park‚ Aintree Avenue‚ Trowbridge‚ Wiltshire‚ UK. BA14 0XB SUMMARY This paper looks at the theoretical development of a natural preservative system using the author’s data base on medicinal plants as a source of references. The legal aspects of this concept are considered. The traditional methods of preservation‚ many taken from the food industry are summarised. The use of alcohol‚ glycerine
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bubble shaped‚ round‚ regular‚ sticky and white coloured colonies were observed. From these initial results‚ the isolated bacteria were tentatively identified as Agrobacterium strains. B.Characterization of Agrobacterium tumefaciens: Biochemical tests: biochemical features of the selected isolates were given in the table 1. The Gram reaction indicates selected isolated are Gram negative. All the six isolated are positive
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Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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