INTRODUCTION Many infectious diseases caused by infectious agents‚ including bacteria‚ fungi‚ viruses and parasites‚ have plagued human existence CITATION Kon97 \l 1033 (Koneman‚ 1997). Thus the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate
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in vitro antimicrobial activity of Sudanese Cucumis melo L. fixed oil was investigated. Agar cup diffusion assay was performed against two fungal species (Candida albicans and Aspergillus niger)‚ two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Ampicillin‚ gentamycin and clotrimazole were used as positive controls. Antimicrobial activity was shown by all the species except E.coli and P. aeruginosa
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the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA
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conclusive‚ the unknown organism labeled Unknown #11 was found to be a member of the family Enterobacteriacea and Genus Serratia marcescens. INTRODUCTION The field of Microbiology is the study of microorganisms‚ including but not limited to bacteria‚ viruses‚ protozoa‚ and fungi. The exact number of identified organisms numbers in the millions. The vast majority of the aforementioned organisms are too small to be seen with the naked eye but have the potential to cause insurmountable harm to
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In general‚ members of the genus Vibrio are Gram-negative‚ motile and most of the members have a single polar flagellum‚ when grown in liquid medium (Kaysner & DePaola‚ 2004). Vibrio cholerae lives naturally in riverine and is the causative agent of cholera outbreaks and epidemics. Vibrio cholerae is comma-shaped. Initial isolates are slightly curved‚ whereas they can appear as straight rods upon laboratory culturing. Vibrio cholerae is a facultative anaerobe and can undergo respiratory and fermentative
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carried out in this report testing for microbial growth against different environmental factors. Good aseptic techniques were used to prevent contamination‚ resulting in a uniform set of results that are in line with the literature. Introduction Bacteria vary greatly in terms of their characteristics and morphology. Colonies can be classified according to their colour‚ form‚ elevation‚ margin and size. Pure cultures of microorganisms are those that are uniform and are of the same descendants of the
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GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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quinoxaline- 2‚ 3-dione -6-sulphonyl benzimidazole (s) have pronounced effect as compared to plain quinoxaline -2‚3-dione against worms‚ the gram positive and gram negative bacteria. Among all the compounds‚ 2‚ 3-dioxo -1‚ 2‚ 3‚ 4-tetrahydroquinoxaline-6-sulphonyl (2-acetyl) benzimidazole have been exhibited good activity in the dose of 20 mg/ml against earth worm‚ bacteria and fungi like standard drug. This may be due to presence acetyl moiety in the benzimidazole ring conjugated with quinoxaline-2‚ 3- dione
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It is regarded as a valuable item of diet and a household remedy. Mangiferin from the leaves has been reported to possess anti-inflammatory‚ diuretic‚ chloretic and cardio tonic activities and displays a high antibacterial activity against gram positive bacteria. Mango leaves were boiled for 5-10 minutes and extract were given to the mice. As the results of the recorded data‚ shows that the calculated F of 3.29 was higher than the table F of 2.8661 at 0.05% level of significance. There is a significant
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staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine-
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