out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify the basic shape and size of the microbe. In this experiment‚ the microbes E. coli‚ Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. These staining techniques are the correct way to identify the shape and size of microbes.
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determine the identity of unknown number 34‚ a gram stain‚ citrate utilization test‚ and a urease detection test. These tests are explained in detail‚ and the method of performing the tests is described. The tests showed that the bacterium was gram-negative bacilli that did not utilize citrate‚ and the ability to hydrolyze urea using urease. These results indicate that the identity of unknown number 34 is Proteus mirabilis. Introduction Proteus mirabilis is a gram-negative bacillus bacterium. It is found
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uses two or more dyes and is used to show morphology and distinguish cell types B. What is the difference between Gram-positive and Gram-negative cell walls? Structure: Have thick layer of peptidoglycan over inner cytoplasmic membrane. lack LPS-lipopolysaccharides Gram negative appear: pink to transfer to red Structure: In gram negative bacteria the peptidoglycan layer is thinner and is located between space of the outer and inner cytoplasmic membrane. cell
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detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then
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survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating at different times. Actimel and Yakult were both able to survive the gastric juice. Actimel produced more colonies
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aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the methyl red test indicated that no mixed acid fermentation occurred. The DNase test was performed and
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Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They are different from one another because
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