Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive
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Bacteria and Archaea The Archaea are presently recognized as one of the two main domains of prokaryotes. The majority of genes that indicate Archaea to be different from Bacteria are for information transfer processes such as DNA replication‚ transcription and translation. Of these‚ DNA replication machinery appears to be most different between the two domains. In terms of transcription‚ the core subunits of the RNA polymerase are the same in Bacteria and Archaea‚ but archaea also contains several
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The word ‘Hypothesis is derived from a Greek word‚ which means ‘to suppose’. It is usually considered as the principal instrument in research. For a researcher it is a formal question that he or she intends to resolve. In this way a hypothesis may be defined as a proposition or a supposition. The main function of hypothesis is to guide the collection and processing of materials and direct the research. Hypothesis is a tentative conclusion. It is facts based theory. A research scholar will analyze
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Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials
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An experiment was conducted to evaluate the biodegradability and the toxicity of vegetable oils in comparison with the reference mineral-based oils. In the research paper produce by Tamada et al. (2012)‚ carried out an investigation on the biodegradability of vegetable oils and the impact of toxicity of vegetable oils to the environment. The experiment was first started off‚ by establishing the concentration of oil to be biodegraded in the soil for the toxicity test. The concentration of oils that
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ENTERICS EMB‚ SSA‚ BSA [pic] Acronym: T LMI CUP MNL GARI ( TSI‚ LIA‚ MIO‚ Indole‚ Citrate‚ Urease‚ PAD‚ Malonate‚ Lactose‚ Glucose‚ Arabinose‚ Rhamnose‚ Inosite) easy steps to identify: *TSI: A/Ag------ Escherichia coli and Klebsiella pneumoniae A/Ag( yellow both slant and butt with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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Introduction Although bacteria is microscopic in size‚ it is largely important in the healthcare field‚ environmental work‚ food preparation‚ as well as many other industries. In particular‚ it is essential that healthcare workers be able to identify the species of bacteria invading a human reservoir in order to prescribe the correct antibiotic that will kill that species. For the purpose of bacteria identification‚ numerous tests have been devised to find out the exact species in question. However
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BACTERIA Identification (Classification I. Domain: Bacteria 1. Gram Negative Proteobacteria (Phylum XII) Non-Proteobacteria 2. Gram Positive LOW G+C (Phylum XIII) HIGH G+C (Phylum XIV) II. Domain: Archaea III. Domain: Eukarya (Gram Negative 1. Bordetella pertussis Pertussis/Whooping Cough 2. Eikenella corrodens Human Bite Injury
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Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial colony was isolated form Mycorrhizal spores‚ and further
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