One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The SIM test‚ which tests for sulfur reduction‚ indole production‚ and motility‚ MRVP which is
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aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant‚ the P. aeruginosa colonies had a filiform appearance on the edges. I think we correctly identified our unknown as P. aeruginosa because we performed several different tests‚ eleven of which helped us identify our organism on the Gram negative chart that was in our lab
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microbial community was very dynamic in response to the changing conditions such as solids reduction and pH. During the SSDML process‚ variation in the pH of the sludge resulted in a succession of neutrophiles by mild acidophilic bacteria followed by acidophilic bacteria. This succession is reflected by the cluster analysis. Lane 1 which shows the profile of the initial sludge had 18 dominant bands. Aeration and decrease in pH by 0.69 units were the main reasons for the disappearance of 8 bands on
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Name SOLUBILITY CURVES Answer the following questions based on the solubility curve below. Which salt is least soluble in water .. at 2O° C? 2. How many grams of potassium chloride can be dissolved in 200 g of water at 80° C? IO 3. At 40° C‚ how much potassium _ __nitrate coin be dissoiu$tl ^n 30D.g of water? ------W- ’1 80 70 ...- O --60 0 5© 40 4. Which salt shows the least change 30 In solubility from 0° - 100° C? 20 10 At 30° C‚ 90 g of sodium
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procedure called gram stain is used in the identification of bacteria. Bacteria can be gram-positive‚ gram-negative‚ or gram-variable. Most bacteria however‚ are usually gram-positive or gram-negative. Gram-positive and gram-negative bacteria react differently to staining because of the differences in their cell walls. Bacterial cell walls contain a polymer called peptidoglycan. Gram-positive bacteria have more of this polymer than gram-negative bacteria. They are also less complex than gram-negative
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The isolated bacteria were named initially as Bacillus bifidus communis‚ which was later renamed to the genus Bifidobacterium. He observed that Bifidobacteria is the dominant in the gut microflora of breast fed infants and also he observed the clinical advantages in using
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boiling point‚ and melting point (1). The IR helps to determine the compounds such as a functional group. In order to analyze the compound‚ IR and melting point work the best together. According to Pub Med the FT-IR spectroscopy can be used to identify bacteria‚ to characterize neoplastic changes from biopsies‚ and to recognize the various forms of arthritis by analysis of synovial fluid (2). The
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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ABSTRACT Purpose: To evaluate the antifungal and cytotoxicity activity of four medicinal plants like Mystroxylon aethiopicum‚ Lonchocarpus capassa‚ Albizia anthelmentica and Myrica salicifolia. Methods: Microdilution method and brine shrimp lethality test were employed to evaluate antifungal and cytotoxicity of plant extracts. Results: Lonchocarpus capassa leaf extracts exhibited antifungal activity against tested fungal strains with MIC range of 0.78 – 3.125 mg/mL with LCLA extract inhibiting C
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phonetically Dictionary - View detailed dictionary 1. article 1. the The purpose of this is knowing the types of bacteria that exist in our environment and diseases caused by them‚ and how treat it. MATERIAL AND METHODS: An unknown bacterium was handed out
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