Nutmeg Isolation of Trimyristin from Nutmeg and preparation of Myristic Acid from Trimyristin by Hydrolysis Introduction Over 40% of medicinal chemicals in the world were developed from isolation of the natural source. These sources were from fungi‚ plants‚ bacteria‚ animals‚ etc. The techniques used to isolate and purify has been by extraction‚ distillation‚ recrystallization‚ and chromatography. In this experiment we will be trying to obtain a pure organic compound from a natural source. Because
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Alkaline Hydrolysis of Crystal Violet in aqueous solution in the presence of a Cationic and an Anionic Surfactant. 3.5.1 THEORY The alkaline hydrolysis of crystal violet‚ a carbocationicdye(CV+) takes place according to CV+ + OH- ↔ CVOH Where CVOH is the colorless carbinol form of the dye. The kinetics of the reaction can be monitored due to the carbocation form of the dye by measuring the decrease in absorbance at the wavelength corresponding to the absorption maximum(λmax). The hydrolysis is carried
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culture Bacillus subtilis‚ Pseudomonal aeruginosa‚ and Escherichia coli‚ to each of the separate sections. The plate was incubated until the next lab period‚ where we then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table 1) * In order to test carbohydrate catabolism under anaerobic conditions we tested cultures of Pseudomonas aerugenosa‚ Escherichia coli‚ and Alcaligenes faecalis in tubes containing a glucose medium. After inoculating each tube with
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more. Hydrolysis is a process that breaks down chains of polymers into monomers. In this experiment‚ we Benedict’s reagent was used both before and after hydrolysis‚ to identify reducing sugars‚ which includes both monosaccharaides and disaccharides. Benedict’s reagent changes color in the presence of reducing sugars as well as heat. Low concentrations of sugar yield a yellow to orange color while higher concentrations will be red to brown. Lugol’s solution was used before and after hydrolysis to
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prepare soap. To compare the properties of soap and synthetic detergents. II. Data and Observation A. Comparison of soaps and detergents 1. Hydrolysis Observation Reaction with blue litmus paper Acetic acid + Red Benzoic acid + Red Sodium Benzoate + Red (+)-miscible (-)-immiscible (red)-acidic (blue)-basic The hydrolysis was tested with acetic acid‚ benzoic acid‚ and sodium benzoate. 2mL water was placed in a test tube and 6 drops of sample was added to each. The mixture
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Chem 31.1 Post Lab 3 CARBONYL COMPOUNDS AND CARBOHYDRATES HYDROLYSIS OF POLYSACCHARIDES - - Susceptible to both nucleophiles and electrophiles Presence of C=O TEST FOR ALDEHYDES AND KETONES - - Reaction with 2‚4-DNP (Dinitrophenylhydrazine) Positive: Formation of yellow precipitate IODOFORM TEST - Carbonyl compounds: Methyl ketones will give a positive result Postive: Yellow precipitate (CHI3) MOLISCH’S TEST for carbohydrates - TOLLEN’S TEST – for aldehydes
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phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline phosphatase by the metal ions was accompanied by changes in the kinetic parameters of pnitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg2+ and Zn2+ ions in promoting the hydrolysis of p-nitrophenylphosphate by alkaline phosphatase. The results show that assays of alkaline phosphatase activity in homogenised tissue samples will give better responses if both Mg2+ and Zn2+
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Chemistry Lab: Hydrolysis of Salts Name_____________________________________________________Date__________________ New Concept: Hydrolysis Reactions Review Concepts/Application: Writing chemical formulas‚ writing ionic equations‚ determining solubility‚ measuring acidity and basicity. Pre-Lab Discussion A salt is an ionic compound containing positive ions other than H+ and negative ions other than OH-. Most salts will dissociate to some degree when placed in water. In many
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should also withstand higher temperatures and lower pH and with minimal by product formation [161]. Fermentation is the key component where advancement in technology plays key role and is required to be feasible. The technologies like Separate hydrolysis and fermentation (SHF)‚ Simultaneous saccharification and fermentation (SSF) and Consolidated bio processing(CBP) are developing. CBP is in the early stages of its development and hence gaining more
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Biofuel from Sago Pith Residue (Sago Hampas) Professors: Student: Student ID: Guo-Jane‚ Tsai Yeuk-Chuen‚ Liu Norhayati Othman 10132067 May 22‚ 2013 1 Outline 1. Introduction 2. Glucose Recovery from Sago Hampas by Three Cycles Hydrolysis for Bioethanol Production. of 3. Conversion of Sago Hampas into Fermentable Sugars Performed Using Cellulolytic Enzymes. 4. Conversion of Fermentable Sugars from Sago Hampas Using UPM2 to Acetone- Butanol- Ethanol (ABE) by Clostridium acetobutylicum
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