In this experiment‚ a reaction is considered where a colorless Paranitrophenol Phosphate (PNPP) is converted to the yellow paranitrophenol (PNP) through the action of an enzyme called alkaline phosphatase. This enzyme normally acts on molecules that have a phosphate group by cleaving it. The main purpose of the experiment is to study the kinetics of alkaline phosphatase when acting on a conversion of PNPP to PNP by measuring
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Acids‚ Bases‚ and Buffers Introduction: The pH scale is used to determine how acidic or basic a solution is‚ ranging from 1-14. The most acidic of all acids are at a pH level of 1 and the most basic of all bases are at 14. The neutral pH level is 7‚ which is what drinking water is. The pH level is determined by the amount of H+ ions present in a solution‚ and the more H+ ions there are the more acidic it is‚ and the lack of these ions results in more basic solutions. One distinguishing feature
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SCH4U 03/04/13 Hydrogen Bonding Purpose: The purpose of this investigation is to test the concept of hydrogen bonding. Hypothesis: Given the concept of hydrogen bonding I predict that the bulky glycerol molecules limits the number of possible hydrogen bonds. If water is mixed with glycerol should make it possible for water to form many hydrogen bonds with the glycerol molecules‚ causing it to become a exothermic reaction. Because glycerol has more possibilities for hydrogen bonding I believe
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The human blood buffer response to increased amounts of acids and bases was determined by titration of a 25.00 mL sample of H2PO4 buffer solution with 0.1 M HCl and 0.1 M NaOH. The volume of 0.1 M HCl is about double of the amount 0.1 M NaOH used to lower/raise the pH of a blood buffer. In this experiment‚ HCl (a strong acid) and NaOH (a strong base) are used as examples of strong acids/bases‚ and the titration with H2PO4 shows the effect on a buffer solution. The assumption was the addition
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Hydrogen Automobiles The effect of pollution that is emitted from vehicles is a serious concern in today ’s society. The cause of this pollution is the toxic emissions from current vehicles. This toxic emissions or pollution destroys the Earth ’s ozone layer or atmospheric gases Acknowledging these facts‚ many are very concerned with the condition of the Earth ’s atmospheric gases in the future. In just a very short time‚ scientists produced a new vehicle which only emits water out of its exhaust
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Acids‚ Bases and Buffers Lab Acids‚ Bases and Buffers Lab Results: The experimental results for part one is as follows: Part One Data Table | Initial pH | Final pH | Test Tube A | 6 | 1 | Test Tube B | 4 | 4 | Test Tube C | 4 | ----- | Test Tube D | 4 | 4 | Test Tube E | 6 | 11 | The experimental results for part two is as follows: Part Two Data Table | Before CO2 was Added | After CO2 was Added | Colour | Blue/green | Light green/yellow | pH Level | 8.0pH | 5.0pH |
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1. Identify the functional group or molecule for each of the following. (3 points) 1. COOH -OH 2. COH -OH 3. COH -OH 1. List whether each of the following substances was positive or negative for reducing sugar‚ as indicated by the Benedict’s test. (6 points) 1. corn syrup (1 point) positive 2. table sugar (1 point) positive 3. unknown 1 (1 point) negative 4. unknown 2 (1 point) negative 5. unknown 3 (1 point) positive 6. unknown 4 (1 point)
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Observing Cells Objectives: After completing this exercise and reading the corresponding material in your text‚ you should be able to 1. Prepare a wet mount slide 2. Identify structures described in this lab on slides 3. Cite examples of the wide diversity of cell types 4. Relate differences in structure among cells to functional differences Introduction Structurally and functionally‚ all living things share one common feature: all living organisms are composed of cells
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Title: Acids‚ Bases & Buffers Objectives: 1. To understand the acid-base chemistry. 2. To prepare and evaluate a buffer system 3. To measure the buffering capacity of two types of isotonic drinks. Introduction: There are acid-forming‚ basic forming and neutral food‚ however the acid or alkaline properties of a food is unable to judge by the actual acidity of the food itself. For example‚ citrus fruits such as lemon are acidic‚ but they are alkaline-forming when we consume and digest it. Therefore
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Buffer Post Lab Calculation 1. We added too much NaOH to our buffer 1 solution. The original pH was 5.32‚ with 10mL of buffer and 10mL of DI water. We added 5mL of NaOH and the pH changed to 12.12. If 5 mL changed the pH by about 7‚ then we predict that about 2 mL of NaOH would change the pH by about 2. This prediction is based on the fact that 2.5mL is half of 5mL‚ and 3.5 is half of 7‚ so adding a little less than 2.5mL would give us a difference of about 2. For the buffer 2 solution‚ the original
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