Chemiluminescence of Luminol Introduction In this experiment‚ luminol was mixed with hydrogen peroxide (an oxidising agent) resulting in chemiluminescence. The experimentis designed to see how the duration of luminescence is affected by varying temperatures. It has been made to be deliberately inaccurate‚ so another objective would be to see whether subjective observations can be improved by repetition and averaging. Chemiluminescence is the production of light from a release of energy in
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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Effect of Substrate Concentration on Catalase Aim: An experiment to find out how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information: Enzymes such as catalase are globular protein molecules with catalytic properties. A catalyst is a substrate which can alter the rate of reaction without itself undergoing any permanent change. As they are not changed
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substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it rise to the surface of the hydrogen peroxide. The time it takes for the filter to rise is an indication of the rate of the rate of enzyme activity. Rate of enzyme activity-distance time Materials: 1. A specific weight enzyme 2. Filter papers 3. 3% Hydrogen Peroxide solution
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Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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between temperature‚ and the rate of enzyme-catalase reaction. Catalase is an enzyme that reduces the amount of activation energy required to break down hydrogen peroxide (H2O2)‚ a by-product of oxygen metabolism‚ into water (H2O) and oxygen (O) (http://www.wisegeek.org/). This reaction is refered to as the enzyme catalyzed reaction. Hydrogen peroxide substrate binds to the active site of the enzyme stressing the bonds‚ ultimately resulting in a release of oxygen and water. An increase in temperature
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break down hydrogen peroxide. In this experiment our professor extracted peroxidase from potato tissue. In order to determine how temperature affects peroxidase we created solutions and measured their absorbance levels after water bath treatments. The more absorbent the solution was the less hydrogen peroxide there was in the solution. This means the peroxidase was able to break down the hydrogen peroxide. The less absorbent the solution the harder it was for peroxidase to break down hydrogen peroxide
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factors help enzymes break down the poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in the hydrogen peroxide. We also tested another enzyme reaction for pH. In this test we learned that high pH equal high reaction rate. The 4 items
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Investigating the Effect of Concentration on the Rate of Enzyme Catalysed Reaction To investigate the effect of concentration on the rate of enzyme catalysed reaction I must know all the factors that affect it in order to investigate in this. Here are all the factors: Temperature- Enzyme like it warm but not too hot. Enzymes are biological catalysts‚ which speed up chemical reactions. They work most efficiently at the optimum point (37°C). If they are below 37°C they will work
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what affects the rate of reaction. We used Hydrogen peroxide to test the rate of reaction‚ with the temperature of this being our variable that we changed. Hydrogen peroxide is a clear‚ colourless liquid which has various amounts of uses within the laboratory‚ industrial purposes and even in our households. It is mainly used for cleaning products and hair dye but is also used for paper making. Hydrogen peroxide is made up of two oxygen atoms and two hydrogen atoms. The chemical formula for this is H₂O₂
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experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined by observing a larger formation of bubbles‚ the visual indicator of hydrogen peroxide being broken down into a non-toxic form of hydrogen and water. This study allowed us to observe first hand catalase can perform further
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