To determine the effects of pH on catalase enzyme activity.Enzyme catalase Lab Statement of the problem : Catalase breaks down H2O2 (hydrogen peroxide) into water and oxygen. H2O2 is a toxic substance formed during metabolism. Hypothesis: To determine the effects of pH on catalase enzyme activity and as you increase the pH the more oxygen that will be produced. Materials: 1. 5 clean test tubes 2. HCI (acid) 3. Dilute HCI 4. Water 5. NaOH (base) Procedure: We start to fill
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simultaneously. Materials: inoculating loop‚ test tube containing 5 ml of sterile saline‚ wash bottle of water‚ plastic API strip‚ Pasteur pipettes‚ mineral oil‚ 10% ferric chloride‚ Bariit’s A and B solutions‚ Kovac’s reagent‚ zinc dust‚ hydrogen peroxide‚ API 20E incubation tray‚ Bunsen burner‚ oxidase reagent and bacteria Methods: The method used for the API (analytical profile index) 20E System was the aseptic technique was used for the inoculating loop to get a unknown colony from the
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Making and Testing for Hydrogen Gas Felicity Tyler Aim: To produce and test for hydrogen gas. Materials: * rubber stopper * dilute hydrochloric acid (HCI) * zinc pieces (Zn) * test-tube rack * matches * dilute sulphuric acid (H2SO4) * 2 cm strips of magnesium ribbon (Mg) * Iron pieces (Fe) * Test-tube Method 1. Test the different metals with the acids by separately combining
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Which of the organisms‚ if any‚ produced measurable acidic byproducts? Both E. coli and S. epidermidis produced measurable acidic byproduct. H.What is the cellular role of catalase? The cellular role of a catalase is to enable the breakdown of hydrogen peroxide into water and molecular oxygen. I.Which of the organisms‚ if any‚ produced catalase? S. epidermidis is the organism that produced catalase. J.Which of the organisms‚ if any‚ produced acetoin S. epidermidis is the organism that produced acetoin
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TITLE Analyzing the Effects of pH‚ Temperature‚ and Concentration on Peroxidase ABSTRACT The purpose of this experiment was to inspect the effect of environmental conditions on hydrogen peroxidase. The three conditions tested were the effect of peroxidase concentration on the rate of the experiment‚ the effect of pH of the rate of peroxidase activity‚ and the effect of temperature on the rate of peroxidase activity. During the lab‚ the lab group tested 7 test tubes‚ including 1 blank‚ with different
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Environmental Variables and their effect on Horseradish Peroxidase Abstract There are several factors that can affect the rate of reaction of peroxidase such as temperature‚ pH‚ concentration of peroxidase present and whether or not it has been boiled. Our experimental data demonstrated that peroxidase activity peaked between 23 degrees Celsius and 32 degrees Celsius. We found the pH to be 7 for optimal activity. As far as the concentration is concerned our results showed that as the concentration
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Experiment 9 Aim A. To investigate the existence of hydrogen bonds between ethanol molecules. B. To measure the strength of hydrogen bond formed between ethanol molecules C. To investigate the formation of hydrogen bonds between molecules of ethyl ethanoate and trichloromethane. D. To measure the strength of hydrogen bond formed between molecules of ethyl ethanoate and trichloromethane. Procedure A. 1. 10 cm3 of ethanol was added into an insulated 50 cm3 beaker by
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Cleaning‚ Disinfection‚ and Sterilization of Medical Equipment Cleaning‚ Disinfection‚ and Sterilization of Medical Equipment Introduction Medical equipments and surgical instruments are examples of devices that are essential to the care of patients; however‚ because they typically are designed for reuse‚ they also can transmit pathogens if any of the steps involved in reprocessing‚ cleaning‚ disinfection‚ or sterilization are inadequate or experience failures. Because the vast majority of pathogens
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Many organisms can decompose hydrogen peroxide (H2O2) enzymatically. Enzymes are globular proteins‚ responsible for most of the chemical activities of living organisms. They act as catalysts‚ substances that speed up chemical reactions without being destroyed or altered during the process. Enzymes are extremely efficient and may be used over and over again. One enzyme may catalyze thousands of reactions every second. At the start of the reaction‚ there is no product‚ and the concentration is the
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Introduction Reactive oxygen species (ROS) include super oxide ion (O2-)‚ hydrogen peroxide (H2O2) and hydroxyl radical (HO- ) are the causative agents to produce oxidative stress. ROS are produced by two independent pathways: mainly the mitochondrial oxidative metabolism and cellular responses to bacterial invasion‚ xenobiotic and cytokines. To counteract the formation of ROS‚ a number of antioxidant proteins have been described. These proteins are superoxide dismutase‚ catalase‚ glutathione peroxidase
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