Purpose The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity. This lab shows how the enzyme decomposes in hydrogen peroxide. Methods and Materials Refer to handout attached to the back of lab Observations Table 1: The mL of oxygen produced with increase of catalase 30secs 60secs 90secs 120secs 150secs 180secs Disks: 2 17ml 16ml 21ml 26ml 31ml 35ml Disks: 4 8ml 19ml 27ml 35ml 44ml 53ml
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decomposes hydrogen peroxide into water and oxygen. Also catalase positive. Enterococcus faecalis doesn’t show produce bubbles in addition of H2O2 which means it doesn’t produce oxygen and is catalase negative. Conclusion/Interpretation: Bubbles appeared after placing H2O2 shows positive reaction to the catalase enzyme. Depending on the strength of the reaction‚ the bubbles may be tiny or relatively large. The primary reaction catalyzed by catalase is the decomposition of hydrogen peroxide to form
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llllllllllllllllllllllllllllllllllllllllllllllllllllllllllll- llllllllllllllllllllllllllllllllllllllllllllllllllldfdkf Determine the presence of catalase enzyme activity using hydrogen peroxide. 1. Test for the presence of catalase in liver. A. Using a knife‚ cut a slice of liver that measures approximately 1 cm by 1 cm and place it on a slide. B. Using a micropipette‚ place a few drops of hydrogen peroxide (H2O2) on top of the liver. C. Observe the solution for a possible reaction‚ indicated by bubbling. Record the results. 2. Test for
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enzyme + 40ml cold distilled water=0units/ml 4. Save undiluted enzyme for parts C-E 5. Place concentrations in ice bath 6. Obtain an additional four 50ml beakers 7. Label beakers 100‚ 75‚ 50‚ and 0 units/per ml 8. Measure 40ml of a 1% hydrogen peroxide solution into each of the four beakers 9. Using forceps drop a 2.1 cm glass fiber filter disc to ½ its diameter in the catalase solution (start with 100units/ml) 10. Leave inside solution for 5 seconds 11. Remove disk and place on a paper
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hypothesis: If the conversion of hydrogen peroxide to water and oxygen by the enzyme catalase is observed ‚ the amount of oxygen generated can be measured and the rate of the enzyme catalyzed reaction can be calculated. The primary reaction catalyzed by catalase is the decomposition of H2O2 to form water and oxygen. ------------------------------------------------- 2H2O2 → 2H2O+O2(gas) ------------------------------------------------- Catalase + Hydrogen Peroxide --> Complex --> Catalase
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Work Plan for Isolation‚ Purification‚ Identification and Starter Culture Activity of Lactoccocus lactis Submitted by: M.Usman Akram B.S. (Hons.) Dairy Technology mh.usman@hotmail.com Mobile : +923217773736 University of Veterinary and Animal Sciences‚ Ravi Campus Pattoki Lactoccocus lactis Classification: Scientific classification | Kingdom: | Bacteria | Division: | Firmicutes | Class: | Bacilli | Order: | Lactobacillales | Family: | Streptococcaceae | Genus:
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Chemiluminescence Professor Stewart CHM 151L-003 Group Members: Melissa Spegal Jessica Buddi March 19‚ 2013 Megan Cochran Professor Stewart Chemiluminescence March 19‚ 2013 Introduction: The objective of this lab was to carry out a systematic set of experiments in order to determine which combination of chemicals produce the brightest and longest lasting light‚ through chemiluminescence. Chemiluminescence can be defined as the emission of light by a chemical reaction that
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heated to a temperature above 70ºC‚ the enzyme will be dead when it is boiled. Based on the effects of the hydroxylamine treated extract‚ the original hypothesis is again accepted. Because the inhibitor‚ hydroxylamine‚ blocked the substrate of hydrogen peroxide from entering the active site‚ the absorbance units decreased compared to the normal extract absorbance units. Further work that would be needed to test explanations would include a more variety of temperatures as well as other enzymes and compare
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removal of xenobiotics. Despite the fact that oxygen is essential for life‚ it can also provoke damaging oxidative events within cells. Oxygen‚ by its transformation to more reactive forms like superoxide radical (O2−•)‚ hydroxyl radical (•OH) and hydrogen peroxide (H2O2) can nick DNA causing mutagenesis and carcinogenesis‚ damage essential enzymes‚ resulting in loss of enzyme activity‚ as well as denature structural proteins and can also provoke uncontrolled chain reactions‚ such as lipid peroxidation
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Crosslinking Agent Markets in China Released On 14th September 2015 Chinas demand for Crosslinking Agent has grown at a fast pace in the past decade. In the next decade‚ both production and demand will continue to grow. The Chinese economy maintains a high speed growth which has been stimulated by the consecutive increases of industrial output‚ imports & exports‚ consumer consumption and capital investment for over two decades. This new study examines Chinas economic trends‚ investment environment
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