Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
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which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The chemical reaction is shown as 2H2O2 = 2H2O + O2 (Keilin and Hartree 397). The reaction involves primarily the adsorption of hydrogen peroxide at the catalase surface. The decomposition of hydrogen peroxide by catalase is regarded as involving
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Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which slowly decreases in the course of an hour. This decrease in
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|the test tube and no bubbles | |hydrogen peroxide‚ because sand does | | | |arose | |not contain any catalysts or enzymes to| | | | | |break down hydrogen peroxide | | |MnO2 |- Reaction
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to maintain the internal environment [2]. Hydrogen peroxide is an oxidizing agent that can be decomposed by catalase [11]‚ the liver is able to decompose hydrogen peroxide enzymatically because it contains catalase [12]. The reaction rate of an enzyme catalysed reaction under the effect of changes in temperature was tested in this experiment. It was hypothesised that the fastest reaction rate would occur a 37 degrees Celsius. Liver and hydrogen peroxide were placed into 4 different test tubes‚ all
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Investigating the effect of different environmental factors on the reaction of liver and hydrogen peroxide Table of Contents 1 Design 3 1.1 Variables 3 1.2 Safety and Environment 3 2 Data Collection and Analysis 3 2.1 Collected Raw Data 3 2.2 Qualitative data 5 2.3 Processed Data 5 2.4 Graph on test tube 2 5 2.5 Graph on test tube 3 6 2.6 Errors 6 3. Conclusion and Evaluation 6 3.1. Conclusion 6 3.2. Evaluation 7 3.2.1. Random Errors 7 3.2.2. Systematic Errors 7 3.3. Improvements
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G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar Solo Cups Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide Hypothesis: 1. If we change the environment via temperature the glow stick will Its intensity will change 2. If hydrogen peroxide is added to a certain food liver then It would bubble 3. If a lactaid enzyme to milk the It would separate Procedure (A): 1. Collect three glow sticks. 2. Boil water in 400
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Materials: * 3% hydrogen peroxide * Manganese dioxide * Fresh or frozen liver * Potato * Ice Equipment: * Fine clean sand * Stirring rod
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