produces hydrogen peroxide‚ which is extremely poisonous. When the catalase comes in contact with the hydrogen peroxide‚ it is broken down into water and oxygen. Aim: The aim is to investigate and determine the effect of change in pH on the activity of the enzymes catalase. Equipment: Test tubes Test tube rack Pipettes Stopwatch Ruler Pen Safety goggles Gloves Materials: Liver pureed 3% of hydrogen peroxide (H2O2) with few drops of detergent Safety: Hydrogen peroxide
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molecules enclosed in the enzyme Catalase: found in every living thing Takes two molecules of hydrogen peroxide and converts it irreversibly to create oxygen gas and water 2H2O2O2+2H2O Question: What variable affects the rate of enzyme catalysis most? Variables Tested: Hydrogen Peroxide concentration‚ yeast concentration‚ heat and pH Materials: 10% glucose mixture 1.5 %‚ 3% and 6% peroxide mixture Yeast Petri dishes Graduated cylinders Glass Beakers (size varies) pH 3.5 solution
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organisms. The rate of enzyme activity is influenced by temperature‚ pH‚ and the presence of inhibitors. Catalase is an enzyme which is produced by every cell to break down hydrogen peroxide. Hydrogen peroxide is a waste product of cellular activity that is poisonous to cells. Catalase speeds up the decomposition of hydrogen peroxide into harmless water and oxygen gas. The purpose of this lab was to determine whether an inhibitor affects the rate of activity for the enzyme catalase. Since catalase
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is the effect of various temperatures‚ 0°C‚ room temperature‚ 37°C‚ 50°C‚ 60°C‚ on the number of oxygen gas bubbles liberated‚ in a decomposition reaction between the enzyme Catalase‚ obtained from crushed mung beans‚ and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins that have a 3-Dimesional shape and contain a region called the
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11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity studies reflect
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Concentration of Hydrogen Peroxide on the change in reaction rate of liver catalase. Hypothesis: Null Hypothesis: If the concentration of the Hydrogen Peroxide is changed then there would be no change in the reaction rate. Alternate Hypothesis 1: I there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will increase. Alternate Hypothesis 2: If there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction
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pipette • Ice bath • 0.1% hydrogen peroxide solution • 3 thermometers • Liver puree • Warm-water bath • 25-mL graduated cylinder • Clock or watch with second hand • 5 50-mL beakers • Paper towels • Filter-paper disks Part A: Observe the Catalase Reaction 1. Put on apron and safety goggles. Use forceps to place a small piece of raw liver in an open petri dish. Use a dropper pipette to put a drop of hydrogen peroxide solution on the liver. CAUTION: Hydrogen peroxide can be irritating to skin
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¬¬¬¬¬¬04/28/2010 Monday & Wednesday 7:40-10:30 Experiment #2 – Kinetics of the Decomposition of Hydrogen Peroxide Introduction: Chemical reactions are dependent upon two factors: temperature and concentrations of substance. We can monitor the rate at which a chemical decomposes or the rate at which a chemical substance appears. In this experiment we will be measuring the rate of decomposition of hydrogen dioxide with the following reaction: 2 H202 (aq) 4 2 H20 (l) + 02 (g) We can trap the oxygen
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reactions. (Evert‚ RF & Eichhorn‚ SE 2013). Enzymes also control plant metabolic processes such as respiration (Evert RF‚ Eichhorn SE & Perry JB 2013). This experiment focuses on the enzyme catalase. Catalase breaks down hydrogen peroxide into water and oxygen. Hydrogen peroxide is a waste product of cell metabolism that can be toxic to the cell (Evert RF‚ Eichhorn SE & Perry JB 2013). The
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Wh Mia Foust Mr. Dunlap Honor Chemistry‚ Per 7 December 21‚ 2010 Glow Sticks Since glow sticks have been invented‚ consumers have wondered how scientists are able to create these magical sticks of light. They work without producing heat. They don’t need a battery or a bulb‚ Magic? This “cool” light is called luminescence. Unlike incandescence‚ which is light from heat energy‚ luminescence can be emitted at normal or lower temperatures. There are several
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