LAB: Substrate Concentration Affecting the Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because
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Abstract In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the
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speeding up reaction in our cells that break down hydrogen peroxide‚ a toxic substance into a harmless substance which is water and oxygen. Hydrogen peroxide (H2O2) is the by-product of many normal cellular respirations mainly in our body for it to function properly. However‚ H2O2 is a toxic substance that can be harmful for the body once it’s not processed properly. If the cell cannot break down H2O2 into a harmless substance such as water and hydrogen‚ we as the multicellular organism will be poisoned
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promotes the conversion of hydrogen peroxide‚ a powerful and potentially harmful oxidizing agent‚ to water and oxygen. Catalase also uses hydrogen peroxide to oxidize toxins including phenols‚ formic acid‚ formaldehyde and alcohols. Catalase is an example of a particularly efficient enzyme. Catalase has one of the highest turnover numbers for all known enzymes (40‚000‚000 molecules/s). This high rate shows an importance for the enzymes capability for detoxifying hydrogen peroxide and preventing the
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speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment. If the speed of decomposition of hydrogen peroxide is related to the temperature then in high temperature it will
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of the most common enzymes. It is found in living organisms and is used to break down hydrogen peroxide. This must happen because hydrogen peroxide is considered toxic to cells in the body. However‚ when catalase is used it breaks it down into hydrogen and oxygen‚ which is much safer to have in the body. A substrate is the material that is being broken down. In this case‚ the substrate would be hydrogen peroxide. The catalase‚ located in the liver‚ is being used to break down the substrate. Problem
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I. Experiment 1 A. Description of Experiment: Three plastic beakers were used for this experiment. In one‚ a 20 mL of 1% hydrogen peroxide solution was made. In order to get this‚ the stock solution‚ which was 3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay
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Purpose: Cells produce toxic wastes‚ in this experiment hydrogen peroxide‚ and without some sort of molecule to break it down the cell will die‚ along with the organism itself. However with the aid of an enzyme‚ catalase‚ hydrogen peroxide is able to be broken down into an intermediate and thereafter harmless substances water and oxygen. The goal of this lab is to measure the reaction rate of this process in different substances such as a liver‚ a vegetable‚ and breast tissue. By using variables
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An investigation to see how much oxygen is given off when different concentrations of catalase are added with hydrogen peroxide. Aim: To see if changing the concentration of catalase (found in celery) with hydrogen peroxide affects the amount of oxygen given of. Background Information: (Hydrogen peroxide - H2O2 1/2O2+H2O) Enzymes: Hundreds of chemical reactions happen simultaneously inside living cells and it’s the job of enzymes to control and regulate the various metabolic
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FHSB1214 Unit description Biology I Year and trimester of study 2015‚ Trimester 1 Title of lab report Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition. Lecturer’s name Norkhalidah Binti Jamali Investigation of the Enzymatic Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Comments are indicated by ‘>>>’ (in detail)‚ ‘???’ (unclear) or ‘xxx’ (wrong concept) FHSB1214 Biology I (201505)-Lab Report Marking Scheme (Practical
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